Difference between revisions of "Part:BBa K1104207:Experience"
(→Results) |
|||
| (7 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| − | We tested the improved promoter: AhpCp1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. | + | This part: AhpCp1 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104207 Part:BBa_K1104207]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae'']. |
| + | |||
| + | [[File: NYMU_47.png|frame|center|'''AhpCp1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104247 Part:BBa_K1104247])''']] | ||
| + | |||
| + | We use the device: AhpCp1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104247 Part:BBa_K1104247]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104207 Part:BBa_K1104207]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104207 Part:BBa_K1104207]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP. | ||
| + | |||
| + | We tested the improved promoter: AhpCp1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing. | ||
| + | |||
==Results== | ==Results== | ||
| − | + | This is the relative promoter strength of AhpC1p(BBa_K1104207) under different H<sub>2</sub>0<sub>2</sub> concentrations tested using AhpC1p+E0840 ([https://parts.igem.org/Part:BBa_K1104247 Part:K1104247]) and found that there is a trend where the higher the H<sub>2</sub>0<sub>2</sub> concentration, the higher the promoter expression in a slight amount: | |
| − | [[File: | + | [[File: cp1.png|frame|center]] |
| − | [[File: NYMU_all1.png|frame|center]] | + | Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101: |
| − | [[File: NYMU_AA.png|frame|center]] | + | [[File: NYMU_all1.png|frame|center|Comparison between ahpC and its improvements. ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104247 Part:BBa_K1104247])]] |
| + | Promoter testing of ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) and its improvements. | ||
| + | [[File: NYMU_AA.png|frame|center]][[File: NYMU_AAAA.png|frame|center]] | ||
| + | |||
==Discussion== | ==Discussion== | ||
Latest revision as of 21:52, 27 October 2013
This part: AhpCp1 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp1 (Part:BBa_K1104207) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].
We use the device: AhpCp1 +E0840 (Part:BBa_K1104247) includes promoter (Part:BBa_K1104207) and Part:BBa_E0840, induce the promoter by different concentrations of H2O2, and identify the strength. After AhpCp1 (Part:BBa_K1104207) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
We tested the improved promoter: AhpCp1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.
Results
This is the relative promoter strength of AhpC1p(BBa_K1104207) under different H202 concentrations tested using AhpC1p+E0840 (Part:K1104247) and found that there is a trend where the higher the H202 concentration, the higher the promoter expression in a slight amount:
Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:
Promoter testing of ahpC promoter (Part:K362001) and its improvements.
Discussion
Applications of BBa_K1104207
User Reviews
UNIQ8b07b2b6c8ecf833-partinfo-00000000-QINU UNIQ8b07b2b6c8ecf833-partinfo-00000001-QINU