Difference between revisions of "Part:BBa K1104205:Experience"
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This part: AhpCp2 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp2 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104205 Part:BBa_K1104205]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae''].
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[[File: NYMU_45.png|frame|center|'''AhpCp2 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104245 Part:BBa_K1104245])''']]
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We use the device: AhpCp2 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104245 Part:BBa_K1104245]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104205 Part:BBa_K1104205]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCp2 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104205 Part:BBa_K1104205]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
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We tested the improved promoter: AhpCp2 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.
  
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We tested the improved promoter: AhpCp2 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols].  This wiki page shows the protocols we used for promoter testing.
 
 
==Results==
 
==Results==
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Here is our result of AhpCp2 testing:
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This is the relative promoter strength of BBa_K1104205 under different H<sub>2</sub>0<sub>2</sub> concentrations tested using BBa_K11042045 (BBa_K1104205 +E0840) and found that there is a trend where the higher the H<sub>2</sub>0<sub>2</sub> concentration, the higher the promoter expression:
 
[[File: NYMU_AhpCp2_strength.png|frame|center]]
 
[[File: NYMU_AhpCp2_strength.png|frame|center]]
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[[File: NYMU_all1.png|frame|center]]
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Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:
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[[File: NYMU_all1.png|frame|center|Comparison between ahpC and its improvements.
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([https://parts.igem.org/wiki/index.php?title=Part:BBa_K Part:BBa_K1104245])]]
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Promoter testing of ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) and its improvements.
 
[[File: NYMU_AA.png|frame|center]]
 
[[File: NYMU_AA.png|frame|center]]
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[[File: NYMU_AAAA.png|frame|center]]
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==Discussion==
 
==Discussion==
  

Latest revision as of 21:29, 27 October 2013

This part: AhpCp2 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCp2 (Part:BBa_K1104205) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].

AhpCp2 +E0840 (Part:BBa_K1104245)

We use the device: AhpCp2 +E0840 (Part:BBa_K1104245) includes promoter (Part:BBa_K1104205) and Part:BBa_E0840, induce the promoter by different concentrations of H2O2, and identify the strength. After AhpCp2 (Part:BBa_K1104205) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.

We tested the improved promoter: AhpCp2 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.

Results

This is the relative promoter strength of BBa_K1104205 under different H202 concentrations tested using BBa_K11042045 (BBa_K1104205 +E0840) and found that there is a trend where the higher the H202 concentration, the higher the promoter expression:

NYMU AhpCp2 strength.png

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:

Comparison between ahpC and its improvements. (Part:BBa_K1104245)

Promoter testing of ahpC promoter (Part:K362001) and its improvements.

NYMU AA.png
NYMU AAAA.png

Discussion

Applications of BBa_K1104205

User Reviews

UNIQ99f756c5b2fbd320-partinfo-00000000-QINU UNIQ99f756c5b2fbd320-partinfo-00000001-QINU