Difference between revisions of "Part:BBa K1139024"
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M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br> | M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br> | ||
| − | This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and <i>lac</i> promoter. The promoter upstream of <i>g2p</i> is altered to <i> | + | This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and <i>lac</i> promoter. The promoter upstream of <i>g2p</i> is altered to <i>tet </i> promoter. A phage particle is formed only when the host cell receives arabinose because <i>g2p</i> (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle. As a reporter, <i>GFP</i> is inserted in the downstream of the <i>lac</i> promoter.<br> |
Latest revision as of 18:39, 27 October 2013
Ptet-M13-Plac-GFP on pSB3
M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter upstream of g2p is altered to tet promoter. A phage particle is formed only when the host cell receives arabinose because g2p (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle. As a reporter, GFP is inserted in the downstream of the lac promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 162
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6088
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 7349