Difference between revisions of "Part:BBa K1139023"
 
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M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br>
 
M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br>
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This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and <i>lac</i> promoter. The promoter upstream of <i>g2p</i> is altered to <i>tet</i> promoter (repressible promoter). A phage particle is not formed with tetR because <i>g2p</i> (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.<br>
+
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and <i>lac</i> promoter. The promoter upstream of <i>g2p</i>(gene 2 protein) is altered to <i>tet</i> promoter (repressible promoter). A phage particle is not formed with tetR because <i>g2p</i> is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.<br>
  
 
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Latest revision as of 18:23, 27 October 2013

K1139023 Ptet-M13 registry page


Ptet-M13 on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter upstream of g2p(gene 2 protein) is altered to tet promoter (repressible promoter). A phage particle is not formed with tetR because g2p is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 60