Difference between revisions of "Part:BBa K1045014"

Latest revision as of 13:47, 27 October 2013

Promoter reverse - Promoter - DarR operator - GFP generator BBa_E0240

This part consists of BBa_K1045011 (a weak, inverse promoter) placed upstream of BBa_K1045013 (a GFP transcription unit harboring the DarR operator downstream of a strong promoter). Since BBa_K1045011 has an inverse orientation compared to BBa_K1045013, the composite part BBa_K1045014 could be used to clone an inverse coding sequence with an inverse RBS behind the inverse promoter BBa_K1045011. This way, you yield a vector from which both, GFP and the gene of interest, might be expressed. Additionally, if the repressor DarR is present, GFP expression might be controlled by DarR.

Note: It might be useful to place a terminator behind the inverse coding sequence to ensure that expression of the inverse coding sequence does not influence the expression of GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1
Illegal NheI site found at 24
Illegal NheI site found at 104
Illegal NheI site found at 127
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 55
Illegal AgeI site found at 963
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 825

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1045014 short</partinfo>
-This part consists of [[Part:BBa_K1045011|BBa_K1045011]] (a weak, inverse promoter) placed upstream of [[Part:BBa_K1045013|BBa_K1045013]] (a GFP transcription unit harboring the DarR operator downstream of a strong promoter). Since [[Part:BBa_K1045011|BBa_K1045011]] has an inverse orientation compared to [[Part:BBa_K1045013|BBa_K10450013]], the composite part [[Part:BBa_K1045014|BBa_K1045014]] could be used to clone an inverse coding sequence with an inverse RBS behind the inverse promoter [[Part:BBa_K1045011|BBa_K1045011]] yielding a vector from which both, GFP and the gene of interest might be expressed. Additionally, if the repressor DarR is present, GFP expression might be controlled by DarR. <br/ >
+This part consists of [[Part:BBa_K1045011|BBa_K1045011]] (a weak, inverse promoter) placed upstream of [[Part:BBa_K1045013|BBa_K1045013]] (a GFP transcription unit harboring the DarR operator downstream of a strong promoter). Since [[Part:BBa_K1045011|BBa_K1045011]] has an inverse orientation compared to [[Part:BBa_K1045013|BBa_K1045013]], the composite part [[Part:BBa_K1045014|BBa_K1045014]] could be used to clone an inverse coding sequence with an inverse RBS behind the inverse promoter [[Part:BBa_K1045011|BBa_K1045011]]. This way, you yield a vector from which both, GFP and the gene of interest, might be expressed. Additionally, if the repressor DarR is present, GFP expression might be controlled by DarR.
 '''Note:''' It might be useful to place a terminator behind the inverse coding sequence to ensure that expression of the inverse coding sequence does not influence the expression of GFP.