Difference between revisions of "Part:BBa K1045014"

 
 
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<partinfo>BBa_K1045014 short</partinfo>
 
<partinfo>BBa_K1045014 short</partinfo>
  
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To be continued
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This part consists of [[Part:BBa_K1045011|BBa_K1045011]] (a weak, inverse promoter) placed upstream of [[Part:BBa_K1045013|BBa_K1045013]] (a GFP transcription unit harboring the DarR operator downstream of a strong promoter). Since [[Part:BBa_K1045011|BBa_K1045011]] has an inverse orientation compared to [[Part:BBa_K1045013|BBa_K1045013]], the composite part [[Part:BBa_K1045014|BBa_K1045014]] could be used to clone an inverse coding sequence with an inverse RBS behind the inverse promoter [[Part:BBa_K1045011|BBa_K1045011]]. This way, you yield a vector from which both, GFP and the gene of interest, might be expressed. Additionally, if the repressor DarR is present, GFP expression might be controlled by DarR.
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'''Note:''' It might be useful to place a terminator behind the inverse coding sequence to ensure that expression of the inverse coding sequence does not influence the expression of GFP.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:47, 27 October 2013

Promoter reverse - Promoter - DarR operator - GFP generator BBa_E0240

This part consists of BBa_K1045011 (a weak, inverse promoter) placed upstream of BBa_K1045013 (a GFP transcription unit harboring the DarR operator downstream of a strong promoter). Since BBa_K1045011 has an inverse orientation compared to BBa_K1045013, the composite part BBa_K1045014 could be used to clone an inverse coding sequence with an inverse RBS behind the inverse promoter BBa_K1045011. This way, you yield a vector from which both, GFP and the gene of interest, might be expressed. Additionally, if the repressor DarR is present, GFP expression might be controlled by DarR.


Note: It might be useful to place a terminator behind the inverse coding sequence to ensure that expression of the inverse coding sequence does not influence the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NheI site found at 24
    Illegal NheI site found at 104
    Illegal NheI site found at 127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal AgeI site found at 963
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 825