Difference between revisions of "Part:BBa K1088023"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1088023 short</partinfo>
 
<partinfo>BBa_K1088023 short</partinfo>
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See BBa_C0012
 
See BBa_C0012
  
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Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.
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Coding sequence for the LacI protein with a LVA degradation tail and without a RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, thereby promoting transcription.
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A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.
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A rapid degradation tail (LVA) has been added to improve the switch time for high to low performance of this part.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 18:26, 26 October 2013

LacI-LVA (part C0012 without barcodes)

See BBa_C0012

Coding sequence for the LacI protein with a LVA degradation tail and without a RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, thereby promoting transcription. A rapid degradation tail (LVA) has been added to improve the switch time for high to low performance of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]