Difference between revisions of "Part:BBa K1045004:Design"

 
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<partinfo>BBa_K1045004 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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To be continued
 
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This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into ''EcoRI'' and ''PstI'' digested '''pSBC13''' backbone.
  
 
===Source===
 
===Source===
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This part was generated by PCR amplification of wild type ''B. subtilis'' chromosomal DNA (strain ''B. subtilis'' 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).
  
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To be continued
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===References===
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(1) Peter Y Watson & Martha J Fedor (2012) “The ''ydaO'' motif is an ATP-sensing riboswitch in ''Bacillus subtilis''“, Nature Chemical Biology No. 8, pp. 963-965
  
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===References===
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(2) James W Nelson, Narasimhan Sudarsan, Kazuhiro Furukawa, Zasha Weinberg, Joy X Wang & Ronald R Breaker (2013) “Riboswitches in eubacteria sense the second messenger c-di-AMP“, Nature Chemical Biology, Advance Online Publication [doi: 10.1038/nchembio.1363]
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(3) Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin & Sigal Ben-Yehuda (2011) “c-di-AMP reports DNA integrity during sporulation in ''Bacillus subtilis''”, EMBO reports Vol. 12 No. 6, pp. 594-601

Latest revision as of 17:03, 26 October 2013

YdaO Riboswitch with native Promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into EcoRI and PstI digested pSBC13 backbone.

Source

This part was generated by PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).

References

(1) Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965

(2) James W Nelson, Narasimhan Sudarsan, Kazuhiro Furukawa, Zasha Weinberg, Joy X Wang & Ronald R Breaker (2013) “Riboswitches in eubacteria sense the second messenger c-di-AMP“, Nature Chemical Biology, Advance Online Publication [doi: 10.1038/nchembio.1363]

(3) Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin & Sigal Ben-Yehuda (2011) “c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis”, EMBO reports Vol. 12 No. 6, pp. 594-601