Difference between revisions of "Part:BBa K1045002:Design"
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===Design Notes===
 
===Design Notes===
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This part was amplified from genomic Bacillus subtilis DNA.
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This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone (see [[Part:BBa_K1045004|BBa_K1045004]]). It was then further ''SpeI'' and ''EcoRI'' digested and ligated with the ''XbaI'' and ''EcoRI'' digested CFP ([[Part:BBa_E0020|BBa_E0020]]). Altogether this composes '''BBa_K1045002'''.
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===Source===
 
===Source===
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This composite part consists of parts either derived from PCR amplification of wild type ''B. subtilis'' chromosomal DNA (strain ''B. subtilis'' 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen) or from the distribution kit 2013. For more information, see "Design Notes" above.
  
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===References===
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(1) Peter Y Watson & Martha J Fedor (2012) “The ''ydaO'' motif is an ATP-sensing riboswitch in ''Bacillus subtilis''“, Nature Chemical Biology No. 8, pp. 963-965
  
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(2) James W Nelson, Narasimhan Sudarsan, Kazuhiro Furukawa, Zasha Weinberg, Joy X Wang & Ronald R Breaker (2013) “Riboswitches in eubacteria sense the second messenger c-di-AMP“, Nature Chemical Biology, Advance Online Publication [doi: 10.1038/nchembio.1363]
  
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===References===
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(3) Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin & Sigal Ben-Yehuda (2011) “c-di-AMP reports DNA integrity during sporulation in ''Bacillus subtilis''”, EMBO reports Vol. 12 No. 6, pp. 594-601
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Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965
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Latest revision as of 16:54, 26 October 2013

CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone (see BBa_K1045004). It was then further SpeI and EcoRI digested and ligated with the XbaI and EcoRI digested CFP (BBa_E0020). Altogether this composes BBa_K1045002.

Source

This composite part consists of parts either derived from PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen) or from the distribution kit 2013. For more information, see "Design Notes" above.

References

(1) Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965

(2) James W Nelson, Narasimhan Sudarsan, Kazuhiro Furukawa, Zasha Weinberg, Joy X Wang & Ronald R Breaker (2013) “Riboswitches in eubacteria sense the second messenger c-di-AMP“, Nature Chemical Biology, Advance Online Publication [doi: 10.1038/nchembio.1363]

(3) Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin & Sigal Ben-Yehuda (2011) “c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis”, EMBO reports Vol. 12 No. 6, pp. 594-601