Difference between revisions of "Part:BBa K1045010:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | For cloning of the inverse RBS '''BBa_K1045010''', hybridization oligos for the RBS [[Part:BBa_B0034|BBa_B0034]] were used. In these hybridization oligos, Prefix and Suffix were switched to yield an inverted RBS. | |
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===Source=== | ===Source=== | ||
− | + | The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of [[Part:BBa_B0034|BBa_B0034]]. | |
===References=== | ===References=== |
Latest revision as of 16:47, 26 October 2013
RBS BBa_B0034 with inversed Pre- and Suffix
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For cloning of the inverse RBS BBa_K1045010, hybridization oligos for the RBS BBa_B0034 were used. In these hybridization oligos, Prefix and Suffix were switched to yield an inverted RBS.
Source
The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of BBa_B0034.