Difference between revisions of "Part:BBa K1139024"

 
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__NOTOC__
 
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<partinfo>BBa_K1139024 short</partinfo>
 
<partinfo>BBa_K1139024 short</partinfo>
  
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TBD
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M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br>
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This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and <i>lac</i> promoter. The promoter upstream of <i>g2p</i> is altered to <i>bad</i> promoter. A phage particle is formed only when the host cell receives arabinose because <i>g2p</i> (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle. As a reporter, <i>GFP</i>. is inserted on the downstream of the <i>lac</i> promoter.<br>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:12, 26 October 2013

Ptet-M13-Plac-GFP on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and lac promoter. The promoter upstream of g2p is altered to bad promoter. A phage particle is formed only when the host cell receives arabinose because g2p (gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle. As a reporter, GFP. is inserted on the downstream of the lac promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 162
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6088
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7349