Difference between revisions of "Part:BBa K1045006:Design"
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===Design Notes===
 
===Design Notes===
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This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested pSBC13 backbone.  
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This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone.
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===Source===
 
===Source===
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This part was generated by PCR amplification of wild type ''B. subtilis'' chromosomal DNA (strain ''B. subtilis'' 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).
  
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To be continued
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===References===
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(1) Peter Y Watson & Martha J Fedor (2012) “The ''ydaO'' motif is an ATP-sensing riboswitch in ''Bacillus subtilis''“, Nature Chemical Biology No. 8, pp. 963-965
  
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===References===
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(2) James W Nelson, Narasimhan Sudarsan, Kazuhiro Furukawa, Zasha Weinberg, Joy X Wang & Ronald R Breaker (2013) “Riboswitches in eubacteria sense the second messenger c-di-AMP“, Nature Chemical Biology, Advance Online Publication [doi: 10.1038/nchembio.1363]
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Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965
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Latest revision as of 14:03, 26 October 2013

YdaO Riboswitch with native Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into EcoRI and PstI digested pSB1C3 backbone.

Source

This part was generated by PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).

References

(1) Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965

(2) James W Nelson, Narasimhan Sudarsan, Kazuhiro Furukawa, Zasha Weinberg, Joy X Wang & Ronald R Breaker (2013) “Riboswitches in eubacteria sense the second messenger c-di-AMP“, Nature Chemical Biology, Advance Online Publication [doi: 10.1038/nchembio.1363]