Difference between revisions of "Part:BBa K1119011"
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<partinfo>BBa_K1119011 short</partinfo>
 
<partinfo>BBa_K1119011 short</partinfo>
  
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Uses constitutive mammalian promoter EF-1alpha (BBa_K1119010), green fluorescence protein (BBa_K648013), and hGH polyadenylation sequence (BBa_K404108)
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This is a mammalian GFP generator used for characterization of our EF-1alpha promoter ([[Part:BBa_K1119010|BBa_K1119010]]). This GFP generator contains the EF-1alpha promoter sequence with GFP reporter in RFC25 format([[Part:BBa_K648013|BBa_K648013]]) and hGH polyA terminator ([[Part:BBa_K404108|BBa_K404108]]).
  
 
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Revision as of 11:59, 26 October 2013

Human Elongation Factor-1alpha Promoter - GFP - hGH polyA tail

This is a mammalian GFP generator used for characterization of our EF-1alpha promoter (BBa_K1119010). This GFP generator contains the EF-1alpha promoter sequence with GFP reporter in RFC25 format(BBa_K648013) and hGH polyA terminator (BBa_K404108).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal AgeI site found at 83
    Illegal AgeI site found at 1067
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1470
    Illegal BsaI.rc site found at 972