Difference between revisions of "Part:BBa K1017401"
 
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The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-2 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.
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The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-1 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.
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[[File:NCTU sRNA1-synthesis process.png|center|600px]]
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Latest revision as of 04:33, 26 October 2013

sRNA+rRBS-1


The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-1 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.

NCTU sRNA1-synthesis process.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 144
    Illegal SpeI site found at 129
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 129
    Illegal NotI site found at 135
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 144
    Illegal SpeI site found at 129
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 144
    Illegal SpeI site found at 129
  • 1000
    COMPATIBLE WITH RFC[1000]