Difference between revisions of "Part:BBa K1189006:Design"
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<partinfo>BBa_K1189006 short</partinfo> | <partinfo>BBa_K1189006 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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| + | Once the individual target sequences were made, we designed new primers to create a target sequence construct with both the target sequences together. We did a KAPA PCR to produce our linear product. The PCR product consisted of the two target sequences and cut sites for EcoR1, Xba1 and Spe1. We then ligated our linear PCR product into a pSB1C3 backbone by switching out RFP. | ||
===Source=== | ===Source=== | ||
| − | + | Primers | |
===References=== | ===References=== | ||
| + | <li>Bogdanove, A. J., Schornack, S., & Lahaye, T. (2010). TAL effectors: finding plant genes for disease and defense. <i>Current Opinion in Plant Biology, 13</i>(4), 394–401. doi:10.1016/j.pbi.2010.04.010 | ||
| + | <li>Mussolino, C., & Cathomen, T. (2012). TALE nucleases: tailored genome engineering made easy. <i>Current Opinion in Biotechnology, 23</i>(5), 644–50. doi:10.1016/j.copbio.2012.01.013 | ||
Latest revision as of 01:40, 26 October 2013
TALEA and TALEB Target
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 213
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Once the individual target sequences were made, we designed new primers to create a target sequence construct with both the target sequences together. We did a KAPA PCR to produce our linear product. The PCR product consisted of the two target sequences and cut sites for EcoR1, Xba1 and Spe1. We then ligated our linear PCR product into a pSB1C3 backbone by switching out RFP.
Source
Primers