Difference between revisions of "Part:BBa K1189004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We designed primers that would produce these TALE A target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone. | |
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===Source=== | ===Source=== | ||
| − | + | The target sequence was amplified with primers from the genome of <i>E. coli</i> using polymerase chain reaction. | |
===References=== | ===References=== | ||
| + | <li>Bogdanove, A. J., Schornack, S., & Lahaye, T. (2010). TAL effectors: finding plant genes for disease and defense. <i>Current Opinion in Plant Biology, 13</i>(4), 394–401. doi:10.1016/j.pbi.2010.04.010 | ||
| + | <li>Mussolino, C., & Cathomen, T. (2012). TALE nucleases: tailored genome engineering made easy. <i>Current Opinion in Biotechnology, 23</i>(5), 644–50. doi:10.1016/j.copbio.2012.01.013 | ||
Latest revision as of 01:37, 26 October 2013
TALEA Target ([A])
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 213
Illegal AgeI site found at 325 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed primers that would produce these TALE A target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone.
Source
The target sequence was amplified with primers from the genome of E. coli using polymerase chain reaction.