Difference between revisions of "Part:BBa K1189022:Design"

 
 
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<partinfo>BBa_K1189022 short</partinfo>
 
<partinfo>BBa_K1189022 short</partinfo>
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===Design Notes===
 
===Design Notes===
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On the iGEM parts registry we found TALA (BBa_K782004) that the Slovenian team synthesized for a previous project. TALEs are very large proteins and take a long time to design and synthesize. Therefore, we decided to use these TALEs to test our system. We also saw this as an opportunity to use and build upon parts made by former iGEM teams. The TALE also had a kozak sequence at the front of the sequence, we used PCR to remove the kozak sequence at the front of the TALE, so it would not inhibit the expression
 
===Source===
 
===Source===
  
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Parts Registry BBa_K782004
  
 
===References===
 
===References===
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<li>Bogdanove, A. J., Schornack, S., & Lahaye, T. (2010). TAL effectors: finding plant genes for disease and defense. <i>Current Opinion in Plant Biology, 13</i>(4), 394–401. doi:10.1016/j.pbi.2010.04.010
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<li>Mussolino, C., & Cathomen, T. (2012). TALE nucleases: tailored genome engineering made easy. <i>Current Opinion in Biotechnology, 23</i>(5), 644–50. doi:10.1016/j.copbio.2012.01.013

Latest revision as of 00:55, 26 October 2013

TALE-A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1696
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2425
    Illegal AgeI site found at 451
    Illegal AgeI site found at 1032
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

On the iGEM parts registry we found TALA (BBa_K782004) that the Slovenian team synthesized for a previous project. TALEs are very large proteins and take a long time to design and synthesize. Therefore, we decided to use these TALEs to test our system. We also saw this as an opportunity to use and build upon parts made by former iGEM teams. The TALE also had a kozak sequence at the front of the sequence, we used PCR to remove the kozak sequence at the front of the TALE, so it would not inhibit the expression

Source

Parts Registry BBa_K782004

References

  • Bogdanove, A. J., Schornack, S., & Lahaye, T. (2010). TAL effectors: finding plant genes for disease and defense. Current Opinion in Plant Biology, 13(4), 394–401. doi:10.1016/j.pbi.2010.04.010
  • Mussolino, C., & Cathomen, T. (2012). TALE nucleases: tailored genome engineering made easy. Current Opinion in Biotechnology, 23(5), 644–50. doi:10.1016/j.copbio.2012.01.013