Difference between revisions of "Part:BBa K1189014:Design"
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<partinfo>BBa_K1189014 short</partinfo> | <partinfo>BBa_K1189014 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part has been designed in the RFC 25 Freiburg fusion backbone and can be used to make fusion proteins. The ATG codon is incorporated in the backbone before the NgoMIV restriction cut site. | |
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===References=== | ===References=== | ||
| + | <li>Litowski, J. R., & Hodges, R. S. (2002). Designing heterodimeric two-stranded alpha-helical coiled-coils. Effects of hydrophobicity and alpha-helical propensity on protein folding, stability, and specificity. <i>The Journal of Biological Chemistry, 277</i>(40), 37272–9. doi:10.1074/jbc.M204257200 | ||
Latest revision as of 00:42, 26 October 2013
GFP fused to an E coil under a lacI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 870
Design Notes
This part has been designed in the RFC 25 Freiburg fusion backbone and can be used to make fusion proteins. The ATG codon is incorporated in the backbone before the NgoMIV restriction cut site.