Difference between revisions of "Part:BBa K1031017"

 
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<partinfo>BBa_K1031017 short</partinfo>
 
<partinfo>BBa_K1031017 short</partinfo>
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<p>For detailed information concerning Band-pass Filter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BandpassFilter">2013 Peking iGEM Band-pass Filter/a></p>
  
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J23114-&#966;R73&#916;
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<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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== '''Characteristic''' ==
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We constructed this circuit to test the inhibition effect of C1 repression circuit. It is composed of constitutive promoter <html><a href="https://parts.igem.org/Part:BBa_J23114">J23114</a></html>, Ribosome Binding Site B0034 and RiboJ enzyme.
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<img src="https://static.igem.org/mediawiki/2013/thumb/3/3c/Peking2013_part_114-phiR73.png/800px-Peking2013_part_114-phiR73.png"
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style="width:600px; margin-left:170px" />
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<p style="text-align:center"><b>Fig 1</b> Construction of J23114-φR73Δ circuit. The orange arrowhead stands for promoter J23114, the green oval represents RBS B0034, φR73Δ shown in brown square.
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== '''Features and Sequence''' ==
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1031017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031017 SequenceAndFeatures</partinfo>
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== '''Usage in Band-pass Filter''' ==
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This circuit is an assistant device aiming at testing the inhibition effect of C1 protein to hybrid promoter. We propose to construct a high fluorescence background level, and when C1 protein is expressed, the fluorescence intensity is supposed to go down if the inhibition circuit really works.
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<html>
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<img src="https://static.igem.org/mediawiki/2013/thumb/8/8c/Peking2013_part_114-phiR73_with…ibition_data.png/800px-Peking2013_part_114-phiR73_with_inhibition_data.png"
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style="width:600px; margin-left:170px" />
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<p style="text-align:center"><b>Fig 3</b> Usage of J23114-φR73Δ circuit in Band-pass Filter. It is co-transformed with C1 inhibition circuit to construct high fluorescence background level.
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Revision as of 12:45, 25 October 2013

J23114-B0034-RiboJ-φR73δ

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter/a>


Characteristic

We constructed this circuit to test the inhibition effect of C1 repression circuit. It is composed of constitutive promoter J23114, Ribosome Binding Site B0034 and RiboJ enzyme.

Fig 1 Construction of J23114-φR73Δ circuit. The orange arrowhead stands for promoter J23114, the green oval represents RBS B0034, φR73Δ shown in brown square. == '''Features and Sequence''' == Sequence and Features BBa_K1031017 SequenceAndFeatures == '''Usage in Band-pass Filter''' == This circuit is an assistant device aiming at testing the inhibition effect of C1 protein to hybrid promoter. We propose to construct a high fluorescence background level, and when C1 protein is expressed, the fluorescence intensity is supposed to go down if the inhibition circuit really works.

Fig 3 Usage of J23114-φR73Δ circuit in Band-pass Filter. It is co-transformed with C1 inhibition circuit to construct high fluorescence background level.