Difference between revisions of "Part:BBa K1084403"

Latest revision as of 10:12, 22 October 2013

promoter3-B0034-RFP-dT

This parts is promoter 3 assembled with mRFP1 expression color generator.

iGEM HokkaidoU Japan 2013 original promoter family is constructed from consensus promoter (BBa_K1084001) by adding mutation at -35 region. Other region is same among family members, and similar with promoter consensus sequence.

Fig. 1 Randomized promoter family sequences

Correspondence of sequence and parts number is below.

  -35        BBa_         t. e. rank
  TTGACA     K1084001     1   
  TAGGTC     K1084002     2
  CTGAAG     K1084003     6
  GGGGTG     K1084004     3
  GAGGAT     K1084005     5
  GCAATA     K1084006     7
  GGGGGG     K1084007     8
  TGTGTG     K1084008     4
  AGTGGG     K1084009     9
  TCTCGG     K1084010     10

t. e. = theoretical transcription efficyency

Promoter transcription efficiency was measured with mRFP1 (BBa_K1084401-K1084410), LacZ and Kanamycine (Km) resistance (BBa_K1084501-K1084505).

Fig. 2 mRFP1 assay result

Fig. 3 β-Galactosidase assay result

Fig. 4 Km resistance assay result

Fig. 5 Comparison of assay results and modeling data

Theoretical promoter modeling also has done (see promoter Background page in HokkaidoU 2013 wiki). According to protein, these promoters show relatively another protein activity revel. Choose your best promoter by Promoter Selector. Plate image below is actual worked Promoter Selector (Km resistance).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 621
Illegal AgeI site found at 733
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1084403 short</partinfo>
-This parts is promoter 1 assembled with mRFP1 expression color generator.
+This parts is promoter 3 assembled with mRFP1 expression color generator.
 iGEM HokkaidoU Japan 2013 original promoter family is constructed from consensus promoter (BBa_K1084001) by adding mutation at -35 region. Other region is same among family members, and similar with promoter consensus sequence.
 https://static.igem.org/mediawiki/2013/c/c1/HokkaidoU2013_promoter_Result-fig1.png
+<div>Fig. 1  Randomized promoter family sequences</div>
 Correspondence of sequence and parts number is below.
@@ Line 22: / Line 23: @@
     TCTCGG     K1084010     10
-t. e. = transcription efficyency
+t. e. = theoretical transcription efficyency
-Promoter transcription efficiency was measured with mRFP1, LacZ and Kanamycine resistance.
+Promoter transcription efficiency was measured with mRFP1 (BBa_K1084401-K1084410), LacZ and Kanamycine (Km) resistance (BBa_K1084501-K1084505).
 https://static.igem.org/mediawiki/2013/7/73/HokkaidoU2013_promoter_Result-fig2.png
 <div>Fig. 2  mRFP1 assay result</div>
 https://static.igem.org/mediawiki/2013/1/1d/HokkaidoU2013_promoter_Result-fig4.png
 <div>Fig. 3  &beta;-Galactosidase assay result</div>
-https://static.igem.org/mediawiki/2013/e/eb/HokkaidoU2013_promoter_Result-fig5.png
+https://static.igem.org/mediawiki/parts/7/72/HokkaidoU_2013_Result-f6_1_1.png
-<div>Fig. 4  Comparison of assay results and modeling data</div>
+<div>Fig. 4  Km resistance assay result</div>
+https://static.igem.org/mediawiki/parts/3/39/HokkaidoU_2013_Result-f7.png
+<div>Fig. 5  Comparison of assay results and modeling data</div>
 Theoretical promoter modeling also has done (see promoter Background page in HokkaidoU 2013 wiki).
 According to protein, these promoters show relatively another protein activity revel.
-Choose your best promoter by promoter optimization kit (POK).
+Choose your best promoter by Promoter Selector.
+Plate image below is actual worked Promoter Selector (Km resistance).
+https://static.igem.org/mediawiki/parts/0/00/HokkaidoU_2013_POK_DEMO_48h_1.png
 <!-- Add more about the biology of this part here