Difference between revisions of "Part:BBa K1045016"
(3 intermediate revisions by 3 users not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
{|width='80%' | {|width='80%' | ||
Line 8: | Line 7: | ||
| width=50% valign='top' style='border: 1px solid black'| | | width=50% valign='top' style='border: 1px solid black'| | ||
<partinfo>BBa_K1045016 short</partinfo> | <partinfo>BBa_K1045016 short</partinfo> | ||
− | * | + | * This composite part is composed of [[Part:BBa_K1045001|BBa_K1045001]], an inverse DarR coding sequence, fused to an inverse terminator, [[Part:BBa_K1045009|BBa_K1045009]]. DarR is a transcriptional repressor binding to the DarR operator sequence. Repression by DarR is enhanced by c-di-AMP. In ''E. coli'', DarR seemed to act as a strong repressor even without c-di-AMP ([http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]). [[Part:BBa_K1045016|BBa_K1045016]] could be cloned downstream of inverse promoter-RBS constructs (compare [[Part:BBa_K1045010|BBa_K1045010]] (inverse RBS) and [[Part:BBa_K1045011|BBa_K1045011]] (inverse promoter)) allowing it to be expressed (for example, see [[Part:BBa_K1045017|BBa_K1045017]]). |
|} | |} |
Latest revision as of 14:12, 19 October 2013
|
Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix
|
Secondary Structure
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]