Difference between revisions of "Part:BBa K1045000"
 
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<partinfo>BBa_K1045000 short</partinfo>
 
<partinfo>BBa_K1045000 short</partinfo>
  
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This part is the binding sequence of the c-di-AMP-dependent regulatory protein DarR. When bound by DarR, it is supposed to prevent the downstream information to be transcribed.
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This part is the binding sequence of the c-di-AMP-dependent regulatory protein DarR. Binding of DarR to the DarR operator prevents transcription of the downstream information.
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The important information: The operator is not in the pSBC13 Vector but instead in the pBluescript -KS plasmid. This plasmid provides an Ampicillin resistance. Furthermore it has got a pUC origin.
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The DarR operator is a 14 bp palindromic DNA sequence discovered in ''Mycobacterium smegmatis''. In ''M. smegmatis'', the DarR operator is found upstream of ''darR'' (auto-regulation) and other genes. The sequence motif of the DarR operator varies slightly dependent on the downstream gene (Zhang ''et al''., 2013).  To create this biobrick, the operator sequence found upstream of ''darR'' was used.
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'''The important information''': The operator is not in the '''pSB1C3''' vector but instead in the '''pBluescript -KS plasmid'''. This plasmid provides an ampicillin resistance. Furthermore, it has got a pUC origin.<br/ >
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(Reference: Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096)
 
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Latest revision as of 12:17, 19 October 2013

DarR operator

This part is the binding sequence of the c-di-AMP-dependent regulatory protein DarR. Binding of DarR to the DarR operator prevents transcription of the downstream information.

The DarR operator is a 14 bp palindromic DNA sequence discovered in Mycobacterium smegmatis. In M. smegmatis, the DarR operator is found upstream of darR (auto-regulation) and other genes. The sequence motif of the DarR operator varies slightly dependent on the downstream gene (Zhang et al., 2013). To create this biobrick, the operator sequence found upstream of darR was used.


The important information: The operator is not in the pSB1C3 vector but instead in the pBluescript -KS plasmid. This plasmid provides an ampicillin resistance. Furthermore, it has got a pUC origin.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]