Difference between revisions of "Part:BBa K1045004:Design"

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===Source===
 
===Source===
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This part was generated by PCR amplification of ''B. subtilis'' chromosomal DNA.
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This part was generated by PCR amplification of wild type ''B. subtilis'' chromosomal DNA (strain ''B. subtilis'' 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).
  
 
===References===
 
===References===
 
Peter Y Watson & Martha J Fedor (2012) “The ''ydaO'' motif is an ATP-sensing riboswitch in ''Bacillus subtilis''“, Nature Chemical Biology No. 8, pp. 963-965
 
Peter Y Watson & Martha J Fedor (2012) “The ''ydaO'' motif is an ATP-sensing riboswitch in ''Bacillus subtilis''“, Nature Chemical Biology No. 8, pp. 963-965

Revision as of 18:16, 17 October 2013

YdaO Riboswitch with native Promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into EcoRI and PstI digested pSBC13 backbone.

Source

This part was generated by PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).

References

Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965