Difference between revisions of "Part:BBa K1073001"
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<partinfo>BBa_K1073001 short</partinfo> | <partinfo>BBa_K1073001 short</partinfo> | ||
| − | This construct is derived from <partinfo>BBa_R0071</partinfo> and <partinfo>BBa_B0032</partinfo>. It combines the inducible promoter of the rhl Quorum sensing system of ''P. aeruginosa'' which is activated by RhlR in combination with N- | + | This construct is derived from <partinfo>BBa_R0071</partinfo> and <partinfo>BBa_B0032</partinfo>. It combines the inducible promoter of the rhl Quorum sensing system of ''P. aeruginosa'' which is activated by RhlR in combination with N-butyryl homoserine lactone (N-butyryl-HSL) with an RBS. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The device can be cloned in front of a desired gene of interest in order to induce its expression by N- | + | The device can be cloned in front of a desired gene of interest in order to induce its expression by N-butyryl-HSL. |
| − | + | The inducible Promotor from ''Pseudomonas aeruginosa'' is activated by RhlR complexed with the specific autoinducer N-butyryl-HSL produced by RhlI. The induction of the promoter in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. For further information of the inducible promoter see <partinfo>BBa_R0071</partinfo>. | |
| − | in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. | + | |
| − | + | ||
Latest revision as of 08:51, 17 October 2013
Inducible promoter of the rhl quorum sensing system with RBS
This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of P. aeruginosa which is activated by RhlR in combination with N-butyryl homoserine lactone (N-butyryl-HSL) with an RBS.
Usage and Biology
The device can be cloned in front of a desired gene of interest in order to induce its expression by N-butyryl-HSL. The inducible Promotor from Pseudomonas aeruginosa is activated by RhlR complexed with the specific autoinducer N-butyryl-HSL produced by RhlI. The induction of the promoter in P. aeruginosa leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. For further information of the inducible promoter see BBa_R0071.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]