Difference between revisions of "Part:BBa K1073001"
 
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<partinfo>BBa_K1073001 short</partinfo>
 
<partinfo>BBa_K1073001 short</partinfo>
  
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This construct is derived from <partinfo>BBa_R0071</partinfo> and <partinfo>BBa_B0032</partinfo>. It combines the inducible promoter of the rhl Quorum sensing system of ''P. aeruginosa'' which is activated by RhlR in combination with N-buturyl homoserine lactone (N-buturyl-HSL) with an RBS.  
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This construct is derived from <partinfo>BBa_R0071</partinfo> and <partinfo>BBa_B0032</partinfo>. It combines the inducible promoter of the rhl Quorum sensing system of ''P. aeruginosa'' which is activated by RhlR in combination with N-butyryl homoserine lactone (N-butyryl-HSL) with an RBS.  
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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The inducible Promotor from ''Pseudomonas aeruginosa'' is activated by RhlR complexed with the specific autoinducer N-buturyl-HSL produced by RhlI. The induction of the promoter  
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The device can be cloned in front of a desired gene of interest in order to induce its expression by N-butyryl-HSL.
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in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003].  
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The inducible Promotor from ''Pseudomonas aeruginosa'' is activated by RhlR complexed with the specific autoinducer N-butyryl-HSL produced by RhlI. The induction of the promoter in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. For further information of the inducible promoter see <partinfo>BBa_R0071</partinfo>.
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The device can be cloned in front of a desired gene of interest in order to induce its expression by N-buturyl-HSL.
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For further information see <partinfo>BBa_R0071</partinfo>.
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Latest revision as of 08:51, 17 October 2013

Inducible promoter of the rhl quorum sensing system with RBS

This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of P. aeruginosa which is activated by RhlR in combination with N-butyryl homoserine lactone (N-butyryl-HSL) with an RBS.


Usage and Biology

The device can be cloned in front of a desired gene of interest in order to induce its expression by N-butyryl-HSL. The inducible Promotor from Pseudomonas aeruginosa is activated by RhlR complexed with the specific autoinducer N-butyryl-HSL produced by RhlI. The induction of the promoter in P. aeruginosa leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. For further information of the inducible promoter see BBa_R0071.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]