Difference between revisions of "Part:BBa K1073001"

Latest revision as of 08:51, 17 October 2013

Inducible promoter of the rhl quorum sensing system with RBS

This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of P. aeruginosa which is activated by RhlR in combination with N-butyryl homoserine lactone (N-butyryl-HSL) with an RBS.

Usage and Biology

The device can be cloned in front of a desired gene of interest in order to induce its expression by N-butyryl-HSL. The inducible Promotor from Pseudomonas aeruginosa is activated by RhlR complexed with the specific autoinducer N-butyryl-HSL produced by RhlI. The induction of the promoter in P. aeruginosa leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. For further information of the inducible promoter see BBa_R0071.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1073001 short</partinfo>
-This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of ''P. aeruginosa'' which is activated by RhlR in combination with N-buturyl homoserine lactone (N-buturyl-HSL) with an RBS. The device can be cloned in front of a desired gene of interest in order to induce its Expression by N-buturyl-HSL.
+This construct is derived from <partinfo>BBa_R0071</partinfo> and <partinfo>BBa_B0032</partinfo>. It combines the inducible promoter of the rhl Quorum sensing system of ''P. aeruginosa'' which is activated by RhlR in combination with N-butyryl homoserine lactone (N-butyryl-HSL) with an RBS.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-The inducible Promotor from ''Pseudomonas aeruginosa'' is induced by RhlR complexed with the specific autoinducer butanoyl-homoserine lactone produced by RhlI. In ''Pseudomonas aeruginosa'' the induction of the promoter leads to activation of transcription of different virulence-associated traits in the cell [Medina 2003].
+The device can be cloned in front of a desired gene of interest in order to induce its expression by N-butyryl-HSL.
-For further Information see BBa_R0071.
+The inducible Promotor from ''Pseudomonas aeruginosa'' is activated by RhlR complexed with the specific autoinducer N-butyryl-HSL produced by RhlI. The induction of the promoter in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003]. For further information of the inducible promoter see <partinfo>BBa_R0071</partinfo>.