Difference between revisions of "Part:BBa K1045012"
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<partinfo>BBa_K1045012 short</partinfo> | <partinfo>BBa_K1045012 short</partinfo> | ||
| − | + | This composite part is composed of a strong promoter ([[Part:BBa_J23110|BBa_J23110]]) derived from the Anderson promoter collection and a downstream sequence of the DarR operator ([[Part:BBa_K1045000|BBa_K1045000]]). It could be placed upstream of a protein coding sequence with an RBS in front of it. If the DarR repressor (see [[Part:BBa_K1045001|BBa_K1045001]]) is available, as well, the expression of this protein coding sequence might be regulated by DarR. | |
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Latest revision as of 14:35, 16 October 2013
Promoter with DarR operator
This composite part is composed of a strong promoter (BBa_J23110) derived from the Anderson promoter collection and a downstream sequence of the DarR operator (BBa_K1045000). It could be placed upstream of a protein coding sequence with an RBS in front of it. If the DarR repressor (see BBa_K1045001) is available, as well, the expression of this protein coding sequence might be regulated by DarR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]