Difference between revisions of "Part:BBa K1031311"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1031311 short</partinfo>
 
<partinfo>BBa_K1031311 short</partinfo>
 +
<html>
 +
<p>For detailed information concerning HbpR, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/HbpR">2013 Peking iGEM Biosensor HbpR</a></p>
 +
 +
<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
 +
</html>
 +
 +
 +
== '''Characterization''' ==
 +
 +
 +
In order to fine-tune the performance of HbpR biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031311 is composed of three elements, the constitutive ''Pc'' promoter <html><a href="https://parts.igem.org/Part:BBa_J23114">J23114</a></html>, coding sequence of HbpR and terminator <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html>. ('''Fig 1''')
 +
 +
<html>
 +
<img src="https://static.igem.org/mediawiki/2013/thumb/3/33/Peking2013_part_114-XylS.png/800px-Peking2013_part_114-XylS.png" style="width:600px; margin-left:170px" />
 +
<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Pm</i>/J23114-XylS. Orange arrowheads represent promoters. RBSs are shown as green ovals and squares in dark red stand for B0015 terminator.
 +
</html>
 +
  
−
114-HbpR-TT
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:44, 14 October 2013

J23114-HbpR-Terminator

For detailed information concerning HbpR, please visit 2013 Peking iGEM Biosensor HbpR


Characterization

In order to fine-tune the performance of HbpR biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031311 is composed of three elements, the constitutive Pc promoter J23114, coding sequence of HbpR and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Pm/J23114-XylS. Orange arrowheads represent promoters. RBSs are shown as green ovals and squares in dark red stand for B0015 terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1735
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 449