Difference between revisions of "Part:BBa K1093006"

 
 
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<partinfo>BBa_K1093006 short</partinfo>
 
<partinfo>BBa_K1093006 short</partinfo>
  
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Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to YFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm. This is N-terminal of sfYFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC).  
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Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to BFP it displays improved stability and stronger yellow fluorescence. Excitation peak: 380 nm Emission peak: 440 nm.  
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This is the N-terminus of sfYFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC). It can be used in conjunction with two versions of superfolderFP C-terminus (BBa_K1093014 and BBa_K1093015) of different specificity to sensitivity ratio (BBa_K1093007 being the more sensitive and BBa_K1093008 the more specific). The 'wild type' C-terminus is not applicable, as it spontaneously recreates the native fluorophore with the N-terminal part (although it can be used as an alternative for classical alpha complementation). The part contains Freiburg prefix (suitable for fusion at the N-terminus).
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==References==
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1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and
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characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006
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Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006
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Sep;24(9):1170. PubMed PMID: 16369541.
 +
 
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2: Zhou J, Lin J, Zhou C, Deng X, Xia B. An improved bimolecular fluorescence
 +
complementation tool based on superfolder green fluorescent protein. Acta Biochim
 +
Biophys Sin (Shanghai). 2011 Mar;43(3):239-44. doi: 10.1093/abbs/gmq128. Epub
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2011 Jan 27. PubMed PMID: 21273204.
 +
 
 +
3: Ottmann C, Weyand M, Wolf A, Kuhlmann J, Ottmann C. Applicability of
 +
superfolder YFP bimolecular fluorescence complementation in vitro. Biol Chem.
 +
2009 Jan;390(1):81-90. doi: 10.1515/BC.2009.008. PubMed PMID: 19007309.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:51, 12 October 2013

sfYFP N-terminal

Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to BFP it displays improved stability and stronger yellow fluorescence. Excitation peak: 380 nm Emission peak: 440 nm. This is the N-terminus of sfYFP, that can be used in Bimolecular Fluorescence Complementation system (BiFC). It can be used in conjunction with two versions of superfolderFP C-terminus (BBa_K1093014 and BBa_K1093015) of different specificity to sensitivity ratio (BBa_K1093007 being the more sensitive and BBa_K1093008 the more specific). The 'wild type' C-terminus is not applicable, as it spontaneously recreates the native fluorophore with the N-terminal part (although it can be used as an alternative for classical alpha complementation). The part contains Freiburg prefix (suitable for fusion at the N-terminus).

References

1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170. PubMed PMID: 16369541.

2: Zhou J, Lin J, Zhou C, Deng X, Xia B. An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta Biochim Biophys Sin (Shanghai). 2011 Mar;43(3):239-44. doi: 10.1093/abbs/gmq128. Epub 2011 Jan 27. PubMed PMID: 21273204.

3: Ottmann C, Weyand M, Wolf A, Kuhlmann J, Ottmann C. Applicability of superfolder YFP bimolecular fluorescence complementation in vitro. Biol Chem. 2009 Jan;390(1):81-90. doi: 10.1515/BC.2009.008. PubMed PMID: 19007309.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13