Difference between revisions of "Part:BBa K1055000:Experience"

Latest revision as of 18:20, 12 October 2013

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Please enter how you used this part and how it worked out.

Applications of BBa_K1055001

When tried to fuse to the CheB signaling protein of E. coli C-terminally, we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG = - 232 kj/mol). Please take a look below:

Hypothetical DNA secondary structure of CheB-mKate fusion protein which might be the reason for continously failing clonation attempts. We derived dG = - 232 kj/mol

User Reviews

UNIQ0fefd6208b365933-partinfo-00000000-QINU UNIQ0fefd6208b365933-partinfo-00000001-QINU

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 how you used this part and how it worked out.
-===Applications of BBa_K1055000===
+===Applications of BBa_K1055001===
+When tried to fuse to the CheB signaling protein of ''E. coli''  C-terminally, we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG =  - 232 kj/mol). Please take a look below:
+[[Image:CMK_scstr.png|center|400px|thumb|Hypothetical DNA secondary structure of CheB-mKate fusion protein which might be the reason for continously failing clonation attempts. We derived dG =  - 232 kj/mol]]
 ===User Reviews===
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 <!-- DON'T DELETE --><partinfo>BBa_K1055000 EndReviews</partinfo>
-'''Characterization LssmOrange'''
-This biobrick did measure up to our expectations as shown in the following data. We also used this promoter to express our PET cleaving enzyme pNB-Est13, which is anchored C-terminal to EstA.(''E. Coli'' membrane anchor protein). AraC-Pbad shows a  low background activity and a good respose to the induction with Arabinose.
-[[Image:Spectra_mKate.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
-[[Image:MKate-LSSmOrange_FRET_Pair.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
-[[Image:mKate_bac.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
-[[Image:Grow_log.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]