Difference between revisions of "Part:BBa K1055001:Experience"

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===Applications of BBa_K1055001===
 
===Applications of BBa_K1055001===
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When tried to fuse to the Tar receptor of ''E. coli''  C-terminally we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG =  - 318 kj/mol). Please take a look below:
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[[Image:mfold_Lssmorange.png|center|400px|thumb|Hypothetical DNA secondary structure of Tar-LssmOrange fusion protein which might be the reason for continously failing clonation attempts. We derived dG =  - 318 kj/mol]]
  
 
===User Reviews===
 
===User Reviews===
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[[Image:LSSmOrange_Diagramm.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
 
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[[Image:Grow_log.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
 
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[[Image:FRET.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
 

Latest revision as of 18:09, 12 October 2013

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Applications of BBa_K1055001

When tried to fuse to the Tar receptor of E. coli C-terminally we encountered major clonation problems since our methods were not able to acces the DNA. After numerous trials and several methods, we performed an "m-fold" analysis of our hypothetical DNA construct and obtained information about a hypothetical secondary DNA structure. It is thermodynamically stable ( We derived dG = - 318 kj/mol). Please take a look below:

Hypothetical DNA secondary structure of Tar-LssmOrange fusion protein which might be the reason for continously failing clonation attempts. We derived dG = - 318 kj/mol

User Reviews

UNIQ8db100aa281fc6af-partinfo-00000000-QINU UNIQ8db100aa281fc6af-partinfo-00000001-QINU