Difference between revisions of "Part:BBa K1071001"
ChristianM (Talk | contribs) |
|||
| Line 4: | Line 4: | ||
Zeocin (Phleomycin D1) intercalates into the DNA. It recruits copper ions as co factor and lead to a double strand break. The gene product She ble binds to Zeocin and blocks its function. It gives prokaryotes and eukaryotes a resistance against Zeocin. | Zeocin (Phleomycin D1) intercalates into the DNA. It recruits copper ions as co factor and lead to a double strand break. The gene product She ble binds to Zeocin and blocks its function. It gives prokaryotes and eukaryotes a resistance against Zeocin. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This Zeocin resistance gene was used in our transfected expression vector to select transfected ''P. tricornutum'' colonies. | |
| + | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1071001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1071001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 18:08, 12 October 2013
ble resistance (eucaryotic)
Zeocin (Phleomycin D1) intercalates into the DNA. It recruits copper ions as co factor and lead to a double strand break. The gene product She ble binds to Zeocin and blocks its function. It gives prokaryotes and eukaryotes a resistance against Zeocin.
Usage and Biology
This Zeocin resistance gene was used in our transfected expression vector to select transfected P. tricornutum colonies.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 273
Illegal NgoMIV site found at 334 - 1000COMPATIBLE WITH RFC[1000]