Difference between revisions of "Part:BBa K1093001"
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Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm | Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm | ||
| − | Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). | + | Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF. |
| + | |||
[[File:Superfolderwykres.jpg|center|594px|border|caption]] | [[File:Superfolderwykres.jpg|center|594px|border|caption]] | ||
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).'' | ''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).'' | ||
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''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).'' | ''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).'' | ||
| − | + | ==References== | |
| + | 1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and | ||
| + | characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 | ||
| + | Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 | ||
| + | Sep;24(9):1170. PubMed PMID: 16369541. | ||
| + | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
sfYFP can be used as reporter. | sfYFP can be used as reporter. | ||
Latest revision as of 18:06, 12 October 2013
sfYFP
Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm
Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF.
Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).
| - | Samples | Arithmetic mean | Standard deviation | ||
|---|---|---|---|---|---|
| sfGFP | 100,60 | 100,37 | 99,02 | 100,00 | 0,85 |
| sfCFP | 17,95 | 18,38 | 17,36 | 17,90 | 0,51 |
| sfBFP | 40,65 | 41,52 | 42,25 | 41,47 | 0,80 |
| sfYFP | 218,87 | 216,51 | 217,87 | 217,75 | 1,19 |
Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).
Also Team Warsaw 2013 measured SYFP2 (BBa_K864100) and compared it with sfYFP.
Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).
| - | Samples | Arithmetic mean | Standard deviation | ||
|---|---|---|---|---|---|
| sfGFP | 100,60 | 100,37 | 99,02 | 100,00 | 0,85 |
| sfYFP | 218,87 | 216,51 | 217,87 | 217,75 | 1,19 |
| SYFP2 | 424,70 | 418,26 | 412,40 | 418,45 | 6,15 |
Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).
References
1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170. PubMed PMID: 16369541.
Usage and Biology
sfYFP can be used as reporter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 13