Difference between revisions of "Part:BBa K1093001"

Latest revision as of 18:06, 12 October 2013

sfYFP

Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm

Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF.

Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

-	Samples			Arithmetic mean	Standard deviation
sfGFP	100,60	100,37	99,02	100,00	0,85
sfCFP	17,95	18,38	17,36	17,90	0,51
sfBFP	40,65	41,52	42,25	41,47	0,80
sfYFP	218,87	216,51	217,87	217,75	1,19

Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

Also Team Warsaw 2013 measured SYFP2 (BBa_K864100) and compared it with sfYFP.

Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

-	Samples			Arithmetic mean	Standard deviation
sfGFP	100,60	100,37	99,02	100,00	0,85
sfYFP	218,87	216,51	217,87	217,75	1,19
SYFP2	424,70	418,26	412,40	418,45	6,15

Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

References

1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170. PubMed PMID: 16369541.

Usage and Biology

sfYFP can be used as reporter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 13

@@ Line 4: / Line 4: @@
 Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm
-Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908).
+Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF.
-[[File:Superfolderwykres.jpg|left|]
+[[File:Superfolderwykres.jpg|center|594px|border|caption]]
 ''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''
@@ Line 47: / Line 48: @@
 Also Team Warsaw 2013 measured SYFP2 (BBa_K864100) and compared it with sfYFP.
-[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]
+[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]
 ''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''
@@ Line 81: / Line 82: @@
 ''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''
-<!-- Add more about the biology of this part here
+==References==
+: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and
+characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006
+Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006
+Sep;24(9):1170. PubMed PMID: 16369541.
 ===Usage and Biology===
 sfYFP can be used as reporter.