Difference between revisions of "Part:BBa K1093001"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1093001 short</partinfo>
 
<partinfo>BBa_K1093001 short</partinfo>
  
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Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eBFP it displays improved stability and strong blue fluorescence.
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Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm
 +
 
 +
Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF.
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[[File:Superfolderwykres.jpg|center|594px|border|caption]]
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''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''
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 +
{| class="wikitable"
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|-
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! scope="col"| -
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! colspan="3" | Samples
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! scope="col" | Arithmetic mean
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! scope="col" | Standard deviation
 +
|-
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|sfGFP
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|100,60
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|100,37
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|99,02
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|100,00
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|0,85
 +
|-
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|sfCFP
 +
|17,95
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|18,38
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|17,36
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|17,90
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|0,51
 +
|-
 +
|sfBFP
 +
|40,65
 +
|41,52
 +
|42,25
 +
|41,47
 +
|0,80
 +
|-
 +
|sfYFP
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|218,87
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|216,51
 +
|217,87
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|217,75
 +
|1,19
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|-
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|}
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''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''
 +
 
 +
Also Team Warsaw 2013 measured SYFP2 (BBa_K864100) and compared it with sfYFP.
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[[File:Sfyfpsyfp2wykres.jpg|center|541px|border|caption]]
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''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''
 +
 
 +
{| class="wikitable"
 +
|-
 +
! scope="col"| -
 +
! colspan="3" | Samples
 +
! scope="col" | Arithmetic mean
 +
! scope="col" | Standard deviation
 +
|-
 +
|sfGFP
 +
|100,60
 +
|100,37
 +
|99,02
 +
|100,00
 +
|0,85
 +
|-
 +
|sfYFP
 +
|218,87
 +
|216,51
 +
|217,87
 +
|217,75
 +
|1,19
 +
|-
 +
|SYFP2
 +
|424,70
 +
|418,26
 +
|412,40
 +
|418,45
 +
|6,15
 +
|-
 +
|}
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''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''
 +
 
 +
==References==
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1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and
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characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006
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Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006
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Sep;24(9):1170. PubMed PMID: 16369541.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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sfYFP can be used as reporter.
  
 
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Latest revision as of 18:06, 12 October 2013

sfYFP

Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm

Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Measurment was conducted on pSB1A3 plasmid with J23100 promoter and B0034 RBS in living cells in RF.

caption

Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

- Samples Arithmetic mean Standard deviation
sfGFP 100,60 100,37 99,02 100,00 0,85
sfCFP 17,95 18,38 17,36 17,90 0,51
sfBFP 40,65 41,52 42,25 41,47 0,80
sfYFP 218,87 216,51 217,87 217,75 1,19

Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

Also Team Warsaw 2013 measured SYFP2 (BBa_K864100) and compared it with sfYFP.

caption

Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

- Samples Arithmetic mean Standard deviation
sfGFP 100,60 100,37 99,02 100,00 0,85
sfYFP 218,87 216,51 217,87 217,75 1,19
SYFP2 424,70 418,26 412,40 418,45 6,15

Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

References

1: Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. Erratum in: Nat Biotechnol. 2006 Sep;24(9):1170. PubMed PMID: 16369541.

Usage and Biology

sfYFP can be used as reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13