Difference between revisions of "Part:BBa K1071014"
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RFP-MTS localized at the membrane with a slight preference for the poles, which in E. coli are enriched in anionic phospholipids. Taken together, we were able to create an additional BioBrick for our toolbox and to improve the in the registry existing RFP.  
 
RFP-MTS localized at the membrane with a slight preference for the poles, which in E. coli are enriched in anionic phospholipids. Taken together, we were able to create an additional BioBrick for our toolbox and to improve the in the registry existing RFP.  
  
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[https://static.igem.org/mediawiki/2013/d/dc/Mts_page.png]
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<html><img src="https://static.igem.org/mediawiki/2013/d/dc/Mts_page.png" alt="MTS" /></html>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 17:38, 12 October 2013

RFP-MTS

RFP (BBa_E1010) with a C-terminal membrane translocation sequence (MTS). The origin is the protein YlxH from the bacterium Geobacillus thermodenitrificans. The MTS has a hydrophobic site and an affinity for membranes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]