Difference between revisions of "Part:BBa K1055000"
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<partinfo>BBa_K1055000 short</partinfo> | <partinfo>BBa_K1055000 short</partinfo> | ||
− | mKate is a fluorophor derived from dsRed. | + | mKate is a fluorophor derived from dsRed and is a FRET partner of LssmOrange [[https://parts.igem.org/Part:BBa_K1055001 BBa_K1055001]] |
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+ | ===Applications of BBa_K1055000=== | ||
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+ | ''Characterization mKate'' | ||
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+ | [[Image:mKate_bac.png|400px|thumb|right|Figure 1. '''Peller of ''E.coli'' Bl21(DE3) cells with expressed mKate. Left side: without UV radiation, right side: with UV radiation ''']] | ||
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+ | mKate is a red fluorescent protein that we want to use as a acceptor for FRET (Figure 3). According to [http://www.evrogen.com/products/basicFPs.shtml Evrogen] mKate has an excitation maximum at 588 nm and an emission maximum at 633 nm. Figure 2 shows that the excitation maximum is at 579 nm and the emission maximum is 611 nm. The emission maximum is big enough to get stimulated by [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1055001 LSSmOrange], the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement. | ||
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+ | [[Image:Spectra_mKate.png|400px|thumb|left|Figure 2. '''Excitation spectrum (dashed line) and emission spectrum (solid line) of mKate with marked maximums''' ]] | ||
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+ | [[Image:MKate-LSSmOrange_FRET_Pair.png|400px|left|thumb|Figure 3. '''Overlay of excitation spectrum (dashed line) and emission spectrum (solid line) of mKate and LSSmOrange ''']] | ||
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Revision as of 17:38, 12 October 2013
mKate is a fluorophor derived from dsRed
mKate is a fluorophor derived from dsRed and is a FRET partner of LssmOrange [BBa_K1055001]
Applications of BBa_K1055000
Characterization mKate
mKate is a red fluorescent protein that we want to use as a acceptor for FRET (Figure 3). According to [http://www.evrogen.com/products/basicFPs.shtml Evrogen] mKate has an excitation maximum at 588 nm and an emission maximum at 633 nm. Figure 2 shows that the excitation maximum is at 579 nm and the emission maximum is 611 nm. The emission maximum is big enough to get stimulated by LSSmOrange, the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]