Difference between revisions of "Part:BBa K1223011"

Latest revision as of 13:50, 12 October 2013

ampR translational unit (ampicilin resistance CDS+promoter)

amppicillin resistance (beta-lactamase) gene in BB format including CDS and promoter.

Design Notes

AmpR gene encodes for the beta-lactamase (EC 3.5.2.6) protein that confers resistance against beta-lactam antibiotics (ampicillin, carbanecillin).

this construct is widely used for selection of transformants. this part contains constitutive promoter, CDS and the stop codon and can be used as a whole to insert a selection marker into composite, larger biobricks or plasmids.

Source

part extracted from pKD4 plasmid by PCR.

References

http://en.wikipedia.org/wiki/Beta-lactamase

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 154

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1223011 short</partinfo>
@@ Line 5: / Line 4: @@
 amppicillin resistance (beta-lactamase) gene in BB format including CDS and promoter.
-<!-- Add more about the biology of this part here
+===Design Notes===
+AmpR gene encodes for the beta-lactamase (EC 3.5.2.6) protein that confers resistance against beta-lactam antibiotics (ampicillin, carbanecillin).
+this construct is widely used for selection of transformants.
+this part contains constitutive promoter, CDS and the stop codon and can be used as a whole to insert a selection marker into composite, larger biobricks or plasmids.
+===Source===
+part extracted from pKD4 plasmid by PCR.
+===References===
+http://en.wikipedia.org/wiki/Beta-lactamase
 ===Usage and Biology===