Difference between revisions of "Part:BBa K1223011:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K1223011 short</partinfo>
 
<partinfo>BBa_K1223011 short</partinfo>
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===Design Notes===
 
===Design Notes===
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used to replace the selection gene of a plasmid.
 
  
 +
AmpR gene encodes for the beta-lactamase (EC 3.5.2.6) protein that confers resistance against beta-lactam antibiotics (ampicillin, carbanecillin).
  
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this construct is widely used for selection of transformants.
 +
this part contains constitutive promoter, CDS and the stop codon and can be used as a whole to insert a selection marker into composite, larger biobricks or plasmids.
  
 
===Source===
 
===Source===
  
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genbank. extracted from pKD4 plasmid.
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part extracted from pKD4 plasmid by PCR.
  
 
===References===
 
===References===
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http://en.wikipedia.org/wiki/Beta-lactamase

Latest revision as of 13:49, 12 October 2013

ampR translational unit (ampicilin resistance CDS+promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 154


Design Notes

AmpR gene encodes for the beta-lactamase (EC 3.5.2.6) protein that confers resistance against beta-lactam antibiotics (ampicillin, carbanecillin).

this construct is widely used for selection of transformants. this part contains constitutive promoter, CDS and the stop codon and can be used as a whole to insert a selection marker into composite, larger biobricks or plasmids.

Source

part extracted from pKD4 plasmid by PCR.

References

http://en.wikipedia.org/wiki/Beta-lactamase