Difference between revisions of "Part:BBa K1159018"

 
m
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1159018 short</partinfo>
 
<partinfo>BBa_K1159018 short</partinfo>
  
x
+
This part codes for a membrane-anchored version of Catechol-2,3-dioxygenase (XylE) for the chassis ''Physcomitrella patens''. Please see subparts for for further description. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 11: Line 10:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1159018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1159018 SequenceAndFeatures</partinfo>
 
+
<html><!--- Please copy this table containing parameters for BBa_ at the end of the parametrs section ahead of the references. ---><style type="text/css">table#AutoAnnotator {border:1px solid black; width:100%; border-collapse:collapse;} th#AutoAnnotatorHeader { border:1px solid black; width:100%; background-color: rgb(221, 221, 221);} td.AutoAnnotator1col { width:100%; border:1px solid black; } span.AutoAnnotatorSequence { font-family:'Courier New', Arial; } td.AutoAnnotatorSeqNum { text-align:right; width:2%; } td.AutoAnnotatorSeqSeq { width:98% } td.AutoAnnotatorSeqFeat1 { width:3% } td.AutoAnnotatorSeqFeat2a { width:27% } td.AutoAnnotatorSeqFeat2b { width:97% } td.AutoAnnotatorSeqFeat3 { width:70% } table.AutoAnnotatorNoBorder { border:0px; width:100%; border-collapse:collapse; } table.AutoAnnotatorWithBorder { border:1px solid black; width:100%; border-collapse:collapse; } td.AutoAnnotatorOuterAmino { border:0px solid black; width:20% } td.AutoAnnotatorInnerAmino { border:1px solid black; width:50% } td.AutoAnnotatorAminoCountingOuter { border:1px solid black; width:40%;  } td.AutoAnnotatorBiochemParOuter { border:1px solid black; width:60%; } td.AutoAnnotatorAminoCountingInner1 { width: 7.5% } td.AutoAnnotatorAminoCountingInner2 { width:62.5% } td.AutoAnnotatorAminoCountingInner3 { width:30% } td.AutoAnnotatorBiochemParInner1 { width: 5% } td.AutoAnnotatorBiochemParInner2 { width:55% } td.AutoAnnotatorBiochemParInner3 { width:40% } td.AutoAnnotatorCodonUsage1 { width: 3% } td.AutoAnnotatorCodonUsage2 { width:14.2% } td.AutoAnnotatorCodonUsage3 { width:13.8% } td.AutoAnnotatorAlignment1 { width: 3% } td.AutoAnnotatorAlignment2 { width: 10% } td.AutoAnnotatorAlignment3 { width: 87% } td.AutoAnnotatorLocalizationOuter {border:1px solid black; width:40%} td.AutoAnnotatorGOOuter {border:1px solid black; width:60%} td.AutoAnnotatorLocalization1 { width: 7.5% } td.AutoAnnotatorLocalization2 { width: 22.5% } td.AutoAnnotatorLocalization3 { width: 70% } td.AutoAnnotatorGO1 { width: 5% } td.AutoAnnotatorGO2 { width: 35% } td.AutoAnnotatorGO3 { width: 60% } td.AutoAnnotatorPredFeat1 { width:3% } td.AutoAnnotatorPredFeat2a { width:27% } td.AutoAnnotatorPredFeat3 { width:70% } div.AutoAnnotator_trans { position:absolute; background:rgb(11,140,143); background-color:rgba(11,140,143, 0.8); height:5px; top:100px; } div.AutoAnnotator_sec_helix { position:absolute; background:rgb(102,0,102); background-color:rgba(102,0,102, 0.8); height:5px; top:110px; } div.AutoAnnotator_sec_strand { position:absolute; background:rgb(245,170,26); background-color:rgba(245,170,26, 1); height:5px; top:110px; } div.AutoAnnotator_acc_buried { position:absolute; background:rgb(89,168,15); background-color:rgba(89,168,15, 0.8); height:5px; top:120px; } div.AutoAnnotator_acc_exposed { position:absolute; background:rgb(0, 0, 255); background-color:rgba(0, 0, 255, 0.8); height:5px; top:120px; } div.AutoAnnotator_dis { position:absolute; text-align:center; font-family:Arial,Helvetica,sans-serif; background:rgb(255, 200, 0); background-color:rgba(255, 200, 0, 1); height:16px; width:16px; top:80px; border-radius:50%; } </style><div id='AutoAnnotator_container_1381244514885'><table id="AutoAnnotator"><tr><!-- Time stamp in ms since 1/1/1970 1381244514885 --><th id="AutoAnnotatorHeader" colspan="2">Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_<!------------------------Enter BioBrick number here------------------------>">BBa_<!------------------------Enter BioBrick number here------------------------></a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0</th></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Nucleotide sequence</strong> in <strong>RFC 25 N-Part</strong> using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)<br><span class="AutoAnnotatorSequence">&nbsp;AACA<u>ATGCCTGGT&nbsp;...&nbsp;CTCTACAAA<i>ACCGGT</i></u></span><br>&nbsp;<strong>ORF</strong> from nucleotide position 5 to 2032 (excluding stop-codon)</td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid sequence:</strong> (RFC 25 scars in shown in bold, other sequence features underlined; both given below)<br><span class="AutoAnnotatorSequence"><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqNum">1&nbsp;<br>101&nbsp;<br>201&nbsp;<br>301&nbsp;<br>401&nbsp;<br>501&nbsp;<br>601&nbsp;</td><td class="AutoAnnotatorSeqSeq">MPGEVAWWRPLFLIALMPIGVLSNAEGDALNT<b>TG</b>NKGVMRPGHVQLRVLDMSKALEHYVELLGLIEMDRDDQGRVYLKAWTEVDKFSLVLREADEPGMDF<br>MGFKVVDEDALRQLERDLMAYGCAVEQLPAGELNSCGRRVRFQAPSGHHFELYADKEYTGKWGLNDVNPEAWPRDLKGMAAVRFDHALMYGDELPATYDL<br>FTKVLGFYLAEQVLDENGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETWEDLLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVF<br>CGGDYNYPDHKPVTWTTDQLGKAIFYHDRILNERFMTVLT<b>TG</b>SA<u>WSHPQFEK</u>G<u>ENLYFQS</u>G<b>TG</b>PGQPPFPPPPPFTPPPPQTPNGASGENSTGAIAGGVA<br>AGAALLFAAPAIGFAWWRRRRPIEA<b>TG</b>GGSGGGSG<b>TG</b>SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTL<br>TYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN<br>FKIRHNIEDGSVQLADHYQQNTPIGDGPVLSPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKTG*</td></tr></table></span></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Sequence features:</strong> (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a">RFC25 scar (shown in bold):&nbsp;</td><td class="AutoAnnotatorSeqFeat3">33 to 34, 341 to 342, 362 to 363, 426 to 427, 436 to 437</td></tr><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a"><i>Strep</i>-tag II:&nbsp;</td><td class="AutoAnnotatorSeqFeat3">345 to 352</td></tr><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a">TEV cleavage site:&nbsp;</td><td class="AutoAnnotatorSeqFeat3">354 to 360</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid composition:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Ala (A)</td><td class="AutoAnnotatorInnerAmino">46 (6.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Arg (R)</td><td class="AutoAnnotatorInnerAmino">30 (4.4%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asn (N)</td><td class="AutoAnnotatorInnerAmino">27 (4.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asp (D)</td><td class="AutoAnnotatorInnerAmino">46 (6.8%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Cys (C)</td><td class="AutoAnnotatorInnerAmino">5 (0.7%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gln (Q)</td><td class="AutoAnnotatorInnerAmino">20 (3.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Glu (E)</td><td class="AutoAnnotatorInnerAmino">43 (6.4%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gly (G)</td><td class="AutoAnnotatorInnerAmino">71 (10.5%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">His (H)</td><td class="AutoAnnotatorInnerAmino">26 (3.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ile (I)</td><td class="AutoAnnotatorInnerAmino">25 (3.7%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Leu (L)</td><td class="AutoAnnotatorInnerAmino">61 (9.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Lys (K)</td><td class="AutoAnnotatorInnerAmino">35 (5.2%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Met (M)</td><td class="AutoAnnotatorInnerAmino">17 (2.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Phe (F)</td><td class="AutoAnnotatorInnerAmino">35 (5.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Pro (P)</td><td class="AutoAnnotatorInnerAmino">41 (6.1%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ser (S)</td><td class="AutoAnnotatorInnerAmino">29 (4.3%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Thr (T)</td><td class="AutoAnnotatorInnerAmino">43 (6.4%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Trp (W)</td><td class="AutoAnnotatorInnerAmino">11 (1.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Tyr (Y)</td><td class="AutoAnnotatorInnerAmino">24 (3.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Val (V)</td><td class="AutoAnnotatorInnerAmino">41 (6.1%)</td></tr></table></td></tr></table></td></tr><tr><td class="AutoAnnotatorAminoCountingOuter"><strong>Amino acid counting</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Total number:</td><td class="AutoAnnotatorAminoCountingInner3">676</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Positively charged (Arg+Lys):</td><td class="AutoAnnotatorAminoCountingInner3">65 (9.6%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Negatively charged (Asp+Glu):</td><td class="AutoAnnotatorAminoCountingInner3">89 (13.2%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Aromatic (Phe+His+Try+Tyr):</td><td class="AutoAnnotatorAminoCountingInner3">96 (14.2%)</td></tr></table></td><td class="AutoAnnotatorBiochemParOuter"><strong>Biochemical parameters</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Atomic composition:</td><td class="AutoAnnotatorBiochemParInner3">C<sub>3370</sub>H<sub>5127</sub>N<sub>911</sub>O<sub>998</sub>S<sub>22</sub></td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Molecular mass [Da]:</td><td class="AutoAnnotatorBiochemParInner3">75077.6</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Theoretical pI:</td><td class="AutoAnnotatorBiochemParInner3">5.53</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td class="AutoAnnotatorBiochemParInner3">96260 / 96573 (all Cys red/ox)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges</strong>&nbsp;<input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot_1381244514885()' value='Show'><span id="hydrophobicity_charge_explanation"></span><div id="hydrophobicity_charge_container" style='display:none'><div id="hydrophobicity_charge_placeholder0" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder1" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder2" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder3" style="width:100%;height:150px"></div></div></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Codon usage</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Organism:</td><td class="AutoAnnotatorCodonUsage3"><i>E. coli</i></td><td class="AutoAnnotatorCodonUsage3"><i>B. subtilis</i></td><td class="AutoAnnotatorCodonUsage3"><i>S. cerevisiae</i></td><td class="AutoAnnotatorCodonUsage3"><i>A. thaliana</i></td><td class="AutoAnnotatorCodonUsage3"><i>P. patens</i></td><td class="AutoAnnotatorCodonUsage3">Mammals</td></tr><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):</td><td class="AutoAnnotatorCodonUsage3">good (0.73)</td><td class="AutoAnnotatorCodonUsage3">good (0.73)</td><td class="AutoAnnotatorCodonUsage3">good (0.65)</td><td class="AutoAnnotatorCodonUsage3">good (0.74)</td><td class="AutoAnnotatorCodonUsage3">excellent (0.82)</td><td class="AutoAnnotatorCodonUsage3">good (0.74)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Alignments</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">SwissProt:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/P06622'>P06622</a> (100% identity on 306 AAs), <a href='http://www.uniprot.org/uniprot/P42212'>P42212</a> (98% identity on 237 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">TrEML:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/D2XKL2'>D2XKL2</a> (100% identity on 306 AAs), <a href='http://www.uniprot.org/uniprot/Q38Q96'>Q38Q96</a> (100% identity on 293 AAs), <a href='http://www.uniprot.org/uniprot/Q7AXV1'>Q7AXV1</a> (100% identity on 306 AAs), <a href='http://www.uniprot.org/uniprot/Q7BH44'>Q7BH44</a> (100% identity on 306 AAs), <a href='http://www.uniprot.org/uniprot/Q9L780'>Q9L780</a> (99% identity on 306 AAs), <a href='http://www.uniprot.org/uniprot/B6UPG7'>B6UPG7</a> (98% identity on 237 AAs), <a href='http://www.uniprot.org/uniprot/Q71RY9'>Q71RY9</a> (98% identity on 237 AAs), <a href='http://www.uniprot.org/uniprot/Q8GHE2'>Q8GHE2</a> (98% identity on 237 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">PDB:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=1mpy'>1mpy</a> (100% identity on 306 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=2y0g'>2y0g</a> (98% identity on 226 AAs)</td></tr></table></td></tr><tr><th id='AutoAnnotatorHeader' colspan="2"><strong>Predictions</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)</th></tr><tr><td class="AutoAnnotatorLocalizationOuter"><strong>Subcellular Localization</strong> (reliability in brackets)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Archaea:</td><td class="AutoAnnotatorLocalization3">cytosol (100%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Bacteria:</td><td class="AutoAnnotatorLocalization3">cytosol (51%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Eukarya:</td><td class="AutoAnnotatorLocalization3">cytosol (40%)</td></tr></table></td><td class="AutoAnnotatorGOOuter"><strong>Gene Ontology</strong> (reliability in brackets)<br><table class="AutoAnnotatorNoBorder"><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Molecular Function Ontology:</td><td class='AutoAnnotatorGO3'> - </td></tr><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Biological Process Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0019439'>GO:0019439</a> (42%), <a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0055114'>GO:0055114</a> (29%)</td></tr><tr><td class='AutoAnnotatorGO1'> </td><td class='AutoAnnotatorGO2'> </td><td class='AutoAnnotatorGO3'>&nbsp;</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Predicted features:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Disulfid bridges:</td><td class="AutoAnnotatorPredFeat3">&nbsp;- </td></tr><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Transmembrane helices:</td><td class="AutoAnnotatorPredFeat3">398 to 415 going inwards</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.<br>If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.</td></tr></table></div><br><!-- IMPORTANT: DON'T REMOVE THIS LINE, OTHERWISE NOT SUPPORTED FOR IE BEFORE 9 --><!--[if lte IE 8]><script language="javascript" type="text/javascript" src="excanvas.min.js"></script><![endif]--><script type='text/javascript' src='http://code.jquery.com/jquery-1.10.0.min.js'></script><script type='text/javascript' src='http://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script><script>function show_or_hide_plot_1381244514885(){hydrophobicity_datapoints = [[2.5,0.12],[3.5,0.10],[4.5,0.24],[5.5,0.14],[6.5,-0.06],[7.5,-1.22],[8.5,-0.82],[9.5,-0.08],[10.5,0.86],[11.5,2.66],[12.5,3.34],[13.5,3.34],[14.5,3.16],[15.5,2.08],[16.5,2.08],[17.5,1.64],[18.5,1.72],[19.5,2.10],[20.5,2.26],[21.5,0.66],[22.5,1.10],[23.5,-0.44],[24.5,-1.28],[25.5,-1.82],[26.5,-0.76],[27.5,-0.36],[28.5,-0.36],[29.5,-0.42],[30.5,0.14],[31.5,-0.30],[32.5,-1.76],[33.5,-1.84],[34.5,-1.78],[35.5,-0.80],[36.5,-0.34],[37.5,-0.54],[38.5,-0.08],[39.5,-0.08],[40.5,-1.56],[41.5,-1.10],[42.5,-0.90],[43.5,0.18],[44.5,-0.64],[45.5,0.84],[46.5,0.76],[47.5,0.76],[48.5,0.38],[49.5,1.12],[50.5,-0.50],[51.5,-0.90],[52.5,0.56],[53.5,-0.52],[54.5,-1.00],[55.5,-0.48],[56.5,-0.00],[57.5,-1.46],[58.5,-0.00],[59.5,1.40],[60.5,1.58],[61.5,1.50],[62.5,3.10],[63.5,1.64],[64.5,1.26],[65.5,0.64],[66.5,-1.02],[67.5,-2.62],[68.5,-2.62],[69.5,-3.70],[70.5,-3.08],[71.5,-3.08],[72.5,-1.54],[73.5,-1.10],[74.5,0.36],[75.5,-0.34],[76.5,0.92],[77.5,-0.10],[78.5,0.02],[79.5,-1.44],[80.5,0.18],[81.5,-0.88],[82.5,-1.48],[83.5,-0.78],[84.5,-0.24],[85.5,-0.32],[86.5,1.22],[87.5,2.76],[88.5,1.30],[89.5,0.76],[90.5,0.36],[91.5,-1.18],[92.5,-2.64],[93.5,-2.06],[94.5,-1.44],[95.5,-1.42],[96.5,-1.42],[97.5,-0.16],[98.5,0.54],[99.5,0.54],[100.5,0.72],[101.5,0.64],[102.5,0.92],[103.5,1.38],[104.5,0.76],[105.5,-0.50],[106.5,-0.42],[107.5,-0.90],[108.5,-0.98],[109.5,-1.18],[110.5,-1.18],[111.5,0.28],[112.5,-0.78],[113.5,-2.44],[114.5,-2.24],[115.5,-0.78],[116.5,-1.16],[117.5,-0.10],[118.5,0.54],[119.5,1.16],[120.5,0.90],[121.5,0.88],[122.5,1.36],[123.5,0.92],[124.5,0.30],[125.5,0.56],[126.5,-0.12],[127.5,-0.60],[128.5,0.02],[129.5,0.02],[130.5,0.02],[131.5,-0.36],[132.5,-0.88],[133.5,-0.30],[134.5,0.32],[135.5,-1.34],[136.5,-1.54],[137.5,-0.54],[138.5,-1.94],[139.5,-1.30],[140.5,-1.10],[141.5,0.16],[142.5,-1.00],[143.5,-0.26],[144.5,-0.90],[145.5,-0.84],[146.5,-1.84],[147.5,-0.96],[148.5,-1.50],[149.5,-0.66],[150.5,-0.28],[151.5,0.72],[152.5,-0.54],[153.5,-0.62],[154.5,-2.08],[155.5,-2.08],[156.5,-2.58],[157.5,-1.96],[158.5,-1.96],[159.5,-1.44],[160.5,-1.26],[161.5,-0.36],[162.5,-0.98],[163.5,-0.90],[164.5,0.12],[165.5,-0.50],[166.5,-1.58],[167.5,-1.58],[168.5,-0.52],[169.5,-1.54],[170.5,-1.16],[171.5,-1.74],[172.5,-1.74],[173.5,-1.34],[174.5,-1.94],[175.5,-1.70],[176.5,-0.42],[177.5,0.64],[178.5,0.24],[179.5,1.86],[180.5,1.04],[181.5,1.22],[182.5,0.16],[183.5,-0.84],[184.5,-1.32],[185.5,0.34],[186.5,0.16],[187.5,0.60],[188.5,1.16],[189.5,0.10],[190.5,-1.36],[191.5,-0.98],[192.5,-1.04],[193.5,-0.60],[194.5,-0.04],[195.5,0.40],[196.5,-1.06],[197.5,0.02],[198.5,0.22],[199.5,0.22],[200.5,-0.30],[201.5,1.24],[202.5,1.24],[203.5,0.60],[204.5,1.30],[205.5,1.82],[206.5,1.74],[207.5,1.34],[208.5,0.72],[209.5,-0.54],[210.5,0.56],[211.5,0.56],[212.5,-0.50],[213.5,-0.50],[214.5,-0.50],[215.5,-1.42],[216.5,-2.32],[217.5,-2.52],[218.5,-0.98],[219.5,0.08],[220.5,-0.54],[221.5,0.16],[222.5,1.82],[223.5,0.82],[224.5,1.22],[225.5,1.76],[226.5,1.06],[227.5,-0.48],[228.5,0.04],[229.5,-1.36],[230.5,-1.90],[231.5,-0.92],[232.5,0.22],[233.5,0.42],[234.5,1.96],[235.5,2.02],[236.5,0.54],[237.5,-0.14],[238.5,-1.40],[239.5,-3.08],[240.5,-2.52],[241.5,-2.78],[242.5,-1.70],[243.5,-1.64],[244.5,-1.50],[245.5,-0.58],[246.5,0.16],[247.5,-0.04],[248.5,-0.04],[249.5,1.36],[250.5,-0.18],[251.5,-0.16],[252.5,-0.90],[253.5,-0.96],[254.5,-2.42],[255.5,-0.96],[256.5,-0.06],[257.5,-0.78],[258.5,0.28],[259.5,1.34],[260.5,-0.12],[261.5,-0.12],[262.5,1.68],[263.5,1.16],[264.5,1.18],[265.5,1.74],[266.5,0.28],[267.5,-0.76],[268.5,-0.76],[269.5,-0.24],[270.5,-0.80],[271.5,0.80],[272.5,0.86],[273.5,0.70],[274.5,-0.34],[275.5,-0.54],[276.5,-2.08],[277.5,-2.08],[278.5,-1.00],[279.5,-1.00],[280.5,-0.74],[281.5,-0.18],[282.5,-0.88],[283.5,-1.78],[284.5,-0.74],[285.5,-0.36],[286.5,0.28],[287.5,1.62],[288.5,1.06],[289.5,-0.16],[290.5,-0.06],[291.5,-0.70],[292.5,-1.96],[293.5,-2.16],[294.5,-2.54],[295.5,-3.08],[296.5,-2.16],[297.5,-0.90],[298.5,0.50],[299.5,1.12],[300.5,1.74],[301.5,0.20],[302.5,-0.62],[303.5,-1.82],[304.5,-2.00],[305.5,-2.24],[306.5,-2.24],[307.5,-2.62],[308.5,-2.70],[309.5,-2.76],[310.5,-1.60],[311.5,-1.04],[312.5,-0.58],[313.5,0.06],[314.5,0.24],[315.5,-1.30],[316.5,-1.86],[317.5,-0.92],[318.5,-0.86],[319.5,-1.50],[320.5,-0.44],[321.5,1.16],[322.5,0.96],[323.5,0.78],[324.5,0.92],[325.5,-0.14],[326.5,-1.94],[327.5,-1.60],[328.5,-0.58],[329.5,-0.64],[330.5,-0.64],[331.5,-0.64],[332.5,-0.98],[333.5,-1.36],[334.5,-0.80],[335.5,0.74],[336.5,2.40],[337.5,1.70],[338.5,1.18],[339.5,1.24],[340.5,0.24],[341.5,-0.16],[342.5,-0.20],[343.5,-0.22],[344.5,-0.78],[345.5,-0.94],[346.5,-2.00],[347.5,-1.26],[348.5,-1.80],[349.5,-1.94],[350.5,-1.70],[351.5,-1.70],[352.5,-2.96],[353.5,-1.50],[354.5,-0.98],[355.5,-0.34],[356.5,-0.34],[357.5,0.20],[358.5,-0.64],[359.5,-0.52],[360.5,-1.16],[361.5,-0.78],[362.5,-0.70],[363.5,-1.32],[364.5,-1.50],[365.5,-1.74],[366.5,-0.86],[367.5,-1.10],[368.5,-0.72],[369.5,-0.72],[370.5,-0.72],[371.5,-1.60],[372.5,-0.72],[373.5,-0.54],[374.5,-0.54],[375.5,-0.54],[376.5,-0.54],[377.5,-1.42],[378.5,-1.98],[379.5,-1.80],[380.5,-1.80],[381.5,-2.18],[382.5,-1.94],[383.5,-0.88],[384.5,-0.90],[385.5,-0.66],[386.5,-0.66],[387.5,-1.28],[388.5,-1.80],[389.5,-1.78],[390.5,-1.78],[391.5,-0.72],[392.5,0.88],[393.5,1.40],[394.5,1.46],[395.5,1.46],[396.5,1.94],[397.5,1.40],[398.5,1.40],[399.5,1.40],[400.5,1.84],[401.5,1.36],[402.5,1.76],[403.5,2.16],[404.5,2.80],[405.5,2.80],[406.5,2.80],[407.5,1.72],[408.5,1.32],[409.5,1.66],[410.5,1.22],[411.5,1.42],[412.5,2.10],[413.5,1.56],[414.5,0.48],[415.5,-0.34],[416.5,-1.80],[417.5,-3.06],[418.5,-3.78],[419.5,-3.92],[420.5,-2.12],[421.5,-1.92],[422.5,-0.66],[423.5,0.10],[424.5,0.34],[425.5,-0.64],[426.5,-0.02],[427.5,-0.54],[428.5,-0.48],[429.5,-0.48],[430.5,-0.48],[431.5,-0.56],[432.5,-0.48],[433.5,-0.54],[434.5,-0.54],[435.5,-0.62],[436.5,-1.24],[437.5,-1.24],[438.5,-1.80],[439.5,-2.42],[440.5,-1.50],[441.5,-0.16],[442.5,-0.22],[443.5,0.40],[444.5,1.94],[445.5,2.02],[446.5,1.14],[447.5,2.18],[448.5,3.02],[449.5,3.02],[450.5,1.48],[451.5,2.56],[452.5,0.96],[453.5,0.12],[454.5,-1.42],[455.5,0.12],[456.5,-1.34],[457.5,-0.72],[458.5,-1.28],[459.5,-1.36],[460.5,-1.64],[461.5,-1.10],[462.5,-0.18],[463.5,0.30],[464.5,1.00],[465.5,-0.26],[466.5,-0.18],[467.5,-1.72],[468.5,-1.64],[469.5,-2.26],[470.5,-1.20],[471.5,-1.26],[472.5,-0.82],[473.5,-0.82],[474.5,-0.90],[475.5,-0.50],[476.5,-0.50],[477.5,0.52],[478.5,-0.18],[479.5,1.16],[480.5,1.30],[481.5,1.94],[482.5,1.04],[483.5,1.68],[484.5,1.04],[485.5,-0.64],[486.5,-0.38],[487.5,-0.56],[488.5,0.42],[489.5,0.18],[490.5,0.78],[491.5,-0.30],[492.5,-0.12],[493.5,-0.20],[494.5,0.96],[495.5,1.00],[496.5,1.18],[497.5,2.08],[498.5,1.18],[499.5,0.08],[500.5,0.14],[501.5,1.12],[502.5,-0.34],[503.5,0.30],[504.5,1.12],[505.5,1.04],[506.5,-0.70],[507.5,-0.26],[508.5,-1.08],[509.5,-2.34],[510.5,-2.82],[511.5,-1.54],[512.5,-2.06],[513.5,-2.44],[514.5,-2.38],[515.5,-2.44],[516.5,-2.26],[517.5,-0.92],[518.5,-1.00],[519.5,-0.52],[520.5,0.54],[521.5,0.36],[522.5,-0.52],[523.5,-0.44],[524.5,-0.36],[525.5,-0.98],[526.5,-0.52],[527.5,-0.90],[528.5,-0.90],[529.5,-1.72],[530.5,-1.60],[531.5,-1.54],[532.5,-0.28],[533.5,0.98],[534.5,1.10],[535.5,0.54],[536.5,-1.06],[537.5,-1.70],[538.5,-2.96],[539.5,-2.44],[540.5,-2.52],[541.5,-1.96],[542.5,-2.78],[543.5,-1.72],[544.5,-2.16],[545.5,-0.54],[546.5,-1.18],[547.5,0.28],[548.5,-0.78],[549.5,-0.16],[550.5,-1.70],[551.5,-1.06],[552.5,-0.86],[553.5,0.68],[554.5,0.06],[555.5,-0.14],[556.5,0.90],[557.5,-0.56],[558.5,-0.64],[559.5,-0.72],[560.5,0.10],[561.5,0.10],[562.5,0.10],[563.5,-0.10],[564.5,-0.10],[565.5,-0.72],[566.5,-2.32],[567.5,-1.70],[568.5,-2.96],[569.5,-1.28],[570.5,0.18],[571.5,0.80],[572.5,0.24],[573.5,0.16],[574.5,0.02],[575.5,-1.44],[576.5,-1.62],[577.5,-1.68],[578.5,-1.16],[579.5,-2.62],[580.5,-2.08],[581.5,-2.46],[582.5,-2.46],[583.5,-1.36],[584.5,-0.92],[585.5,0.14],[586.5,1.16],[587.5,2.22],[588.5,0.68],[589.5,0.16],[590.5,-1.44],[591.5,-2.60],[592.5,-3.66],[593.5,-3.04],[594.5,-1.36],[595.5,-1.44],[596.5,0.18],[597.5,0.18],[598.5,0.82],[599.5,-0.86],[600.5,0.82],[601.5,-0.92],[602.5,-0.86],[603.5,-2.12],[604.5,-0.44],[605.5,-2.04],[606.5,-1.84],[607.5,-1.28],[608.5,-0.74],[609.5,-0.80],[610.5,-0.80],[611.5,0.66],[612.5,1.10],[613.5,0.56],[614.5,-0.92],[615.5,-0.48],[616.5,-1.94],[617.5,-3.00],[618.5,-3.00],[619.5,-2.50],[620.5,-2.56],[621.5,-0.96],[622.5,-0.34],[623.5,-0.34],[624.5,-0.28],[625.5,-0.28],[626.5,-0.34],[627.5,0.50],[628.5,1.04],[629.5,0.80],[630.5,0.42],[631.5,-1.12],[632.5,-2.52],[633.5,-2.62],[634.5,-1.54],[635.5,-1.00],[636.5,-0.44],[637.5,-0.50],[638.5,-0.40],[639.5,-0.80],[640.5,0.12],[641.5,0.10],[642.5,0.02],[643.5,-0.52],[644.5,-1.20],[645.5,-2.66],[646.5,-3.20],[647.5,-3.20],[648.5,-3.40],[649.5,-3.78],[650.5,-3.72],[651.5,-2.64],[652.5,-1.02],[653.5,0.64],[654.5,2.10],[655.5,2.04],[656.5,2.22],[657.5,2.22],[658.5,1.32],[659.5,0.92],[660.5,1.98],[661.5,1.34],[662.5,1.40],[663.5,1.40],[664.5,0.40],[665.5,-0.04],[666.5,0.42],[667.5,-1.18],[668.5,-1.74],[669.5,-0.34],[670.5,-0.52],[671.5,-1.68],[672.5,-1.12],[673.5,-0.50]];charge_datapoints = [[2.5,-0.20],[3.5,-0.20],[4.5,-0.20],[5.5,-0.20],[6.5,0.20],[7.5,0.20],[8.5,0.20],[9.5,0.20],[10.5,0.20],[11.5,0.00],[12.5,0.00],[13.5,0.00],[14.5,0.00],[15.5,0.00],[16.5,0.00],[17.5,0.00],[18.5,0.00],[19.5,0.00],[20.5,0.00],[21.5,0.00],[22.5,0.00],[23.5,-0.20],[24.5,-0.20],[25.5,-0.40],[26.5,-0.40],[27.5,-0.40],[28.5,-0.20],[29.5,-0.20],[30.5,0.00],[31.5,0.00],[32.5,0.00],[33.5,0.20],[34.5,0.20],[35.5,0.20],[36.5,0.20],[37.5,0.40],[38.5,0.20],[39.5,0.20],[40.5,0.30],[41.5,0.30],[42.5,0.10],[43.5,0.10],[44.5,0.30],[45.5,0.20],[46.5,0.20],[47.5,0.00],[48.5,0.00],[49.5,-0.20],[50.5,0.00],[51.5,0.00],[52.5,0.20],[53.5,0.00],[54.5,0.10],[55.5,-0.10],[56.5,-0.10],[57.5,-0.30],[58.5,-0.10],[59.5,-0.20],[60.5,-0.20],[61.5,-0.20],[62.5,0.00],[63.5,-0.20],[64.5,-0.20],[65.5,-0.40],[66.5,-0.20],[67.5,-0.40],[68.5,-0.40],[69.5,-0.40],[70.5,-0.20],[71.5,-0.20],[72.5,0.00],[73.5,0.20],[74.5,0.20],[75.5,0.40],[76.5,0.20],[77.5,0.20],[78.5,0.20],[79.5,0.00],[80.5,-0.20],[81.5,-0.40],[82.5,-0.20],[83.5,-0.20],[84.5,0.00],[85.5,0.00],[86.5,0.20],[87.5,0.00],[88.5,0.20],[89.5,0.00],[90.5,0.00],[91.5,-0.20],[92.5,-0.40],[93.5,-0.60],[94.5,-0.40],[95.5,-0.40],[96.5,-0.40],[97.5,-0.20],[98.5,-0.20],[99.5,-0.20],[100.5,-0.20],[101.5,0.20],[102.5,0.20],[103.5,0.20],[104.5,-0.00],[105.5,-0.20],[106.5,-0.60],[107.5,-0.60],[108.5,-0.60],[109.5,-0.20],[110.5,-0.00],[111.5,0.20],[112.5,-0.00],[113.5,0.20],[114.5,-0.20],[115.5,-0.20],[116.5,-0.20],[117.5,-0.00],[118.5,-0.20],[119.5,-0.00],[120.5,-0.00],[121.5,-0.00],[122.5,-0.00],[123.5,-0.20],[124.5,-0.20],[125.5,-0.20],[126.5,-0.20],[127.5,-0.20],[128.5,-0.00],[129.5,-0.20],[130.5,-0.20],[131.5,-0.20],[132.5,-0.20],[133.5,-0.20],[134.5,-0.00],[135.5,0.20],[136.5,0.40],[137.5,0.40],[138.5,0.60],[139.5,0.60],[140.5,0.40],[141.5,0.20],[142.5,0.20],[143.5,-0.00],[144.5,-0.00],[145.5,0.10],[146.5,0.20],[147.5,0.20],[148.5,-0.00],[149.5,-0.00],[150.5,-0.10],[151.5,-0.20],[152.5,-0.40],[153.5,-0.00],[154.5,-0.20],[155.5,-0.20],[156.5,-0.20],[157.5,-0.00],[158.5,-0.00],[159.5,0.20],[160.5,0.20],[161.5,0.20],[162.5,0.20],[163.5,-0.20],[164.5,-0.20],[165.5,-0.20],[166.5,-0.20],[167.5,-0.40],[168.5,-0.20],[169.5,-0.20],[170.5,-0.20],[171.5,-0.00],[172.5,-0.00],[173.5,-0.00],[174.5,0.20],[175.5,0.20],[176.5,-0.00],[177.5,0.20],[178.5,0.20],[179.5,-0.00],[180.5,0.20],[181.5,0.20],[182.5,-0.00],[183.5,0.10],[184.5,0.10],[185.5,-0.10],[186.5,-0.10],[187.5,0.10],[188.5,-0.00],[189.5,-0.20],[190.5,-0.40],[191.5,-0.40],[192.5,-0.40],[193.5,-0.40],[194.5,-0.20],[195.5,-0.00],[196.5,-0.20],[197.5,-0.20],[198.5,-0.20],[199.5,-0.20],[200.5,-0.00],[201.5,0.20],[202.5,0.20],[203.5,0.20],[204.5,0.20],[205.5,-0.00],[206.5,-0.00],[207.5,-0.00],[208.5,-0.20],[209.5,-0.20],[210.5,-0.20],[211.5,-0.20],[212.5,-0.40],[213.5,-0.40],[214.5,-0.40],[215.5,-0.40],[216.5,-0.40],[217.5,-0.00],[218.5,0.20],[219.5,0.20],[220.5,0.20],[221.5,0.20],[222.5,-0.00],[223.5,-0.00],[224.5,-0.00],[225.5,-0.00],[226.5,-0.00],[227.5,0.20],[228.5,0.20],[229.5,0.30],[230.5,0.10],[231.5,0.10],[232.5,-0.10],[233.5,-0.10],[234.5,-0.20],[235.5,0.10],[236.5,0.20],[237.5,0.20],[238.5,-0.00],[239.5,0.20],[240.5,0.10],[241.5,0.20],[242.5,0.20],[243.5,0.50],[244.5,0.40],[245.5,0.40],[246.5,0.20],[247.5,0.20],[248.5,0.20],[249.5,0.10],[250.5,-0.10],[251.5,-0.10],[252.5,-0.10],[253.5,-0.40],[254.5,-0.60],[255.5,-0.40],[256.5,-0.40],[257.5,-0.20],[258.5,-0.00],[259.5,0.20],[260.5,-0.00],[261.5,-0.00],[262.5,-0.20],[263.5,-0.20],[264.5,-0.20],[265.5,-0.00],[266.5,-0.20],[267.5,-0.20],[268.5,-0.20],[269.5,-0.20],[270.5,-0.40],[271.5,-0.20],[272.5,-0.20],[273.5,-0.20],[274.5,-0.20],[275.5,0.20],[276.5,0.30],[277.5,0.30],[278.5,0.30],[279.5,0.30],[280.5,0.20],[281.5,0.10],[282.5,0.30],[283.5,0.30],[284.5,0.30],[285.5,0.20],[286.5,0.20],[287.5,-0.00],[288.5,-0.20],[289.5,-0.20],[290.5,-0.20],[291.5,-0.20],[292.5,-0.20],[293.5,0.20],[294.5,0.20],[295.5,-0.00],[296.5,-0.00],[297.5,-0.00],[298.5,-0.20],[299.5,-0.20],[300.5,-0.00],[301.5,-0.20],[302.5,-0.20],[303.5,-0.20],[304.5,-0.20],[305.5,-0.20],[306.5,-0.20],[307.5,-0.10],[308.5,0.10],[309.5,0.10],[310.5,0.10],[311.5,0.30],[312.5,0.20],[313.5,-0.00],[314.5,-0.00],[315.5,-0.20],[316.5,-0.20],[317.5,-0.20],[318.5,-0.20],[319.5,-0.00],[320.5,0.20],[321.5,0.20],[322.5,0.20],[323.5,0.20],[324.5,0.10],[325.5,-0.10],[326.5,0.10],[327.5,0.10],[328.5,0.10],[329.5,-0.00],[330.5,-0.00],[331.5,-0.00],[332.5,-0.00],[333.5,-0.00],[334.5,-0.00],[335.5,0.20],[336.5,-0.00],[337.5,-0.00],[338.5,-0.00],[339.5,-0.00],[340.5,-0.00],[341.5,-0.00],[342.5,-0.00],[343.5,-0.00],[344.5,0.10],[345.5,0.10],[346.5,0.10],[347.5,0.10],[348.5,-0.10],[349.5,-0.00],[350.5,-0.00],[351.5,-0.20],[352.5,-0.20],[353.5,-0.00],[354.5,-0.20],[355.5,-0.20],[356.5,-0.00],[357.5,-0.00],[358.5,-0.00],[359.5,-0.00],[360.5,-0.00],[361.5,-0.00],[362.5,-0.00],[363.5,-0.00],[364.5,-0.00],[365.5,-0.00],[366.5,-0.00],[367.5,-0.00],[368.5,-0.00],[369.5,-0.00],[370.5,-0.00],[371.5,-0.00],[372.5,-0.00],[373.5,-0.00],[374.5,-0.00],[375.5,-0.00],[376.5,-0.00],[377.5,-0.00],[378.5,-0.00],[379.5,-0.00],[380.5,-0.00],[381.5,-0.00],[382.5,-0.00],[383.5,-0.00],[384.5,-0.00],[385.5,-0.00],[386.5,-0.20],[387.5,-0.20],[388.5,-0.20],[389.5,-0.20],[390.5,-0.20],[391.5,-0.00],[392.5,-0.00],[393.5,-0.00],[394.5,-0.00],[395.5,-0.00],[396.5,-0.00],[397.5,-0.00],[398.5,-0.00],[399.5,-0.00],[400.5,-0.00],[401.5,-0.00],[402.5,-0.00],[403.5,-0.00],[404.5,-0.00],[405.5,-0.00],[406.5,-0.00],[407.5,-0.00],[408.5,-0.00],[409.5,-0.00],[410.5,-0.00],[411.5,-0.00],[412.5,-0.00],[413.5,-0.00],[414.5,-0.00],[415.5,0.20],[416.5,0.40],[417.5,0.60],[418.5,0.80],[419.5,0.80],[420.5,0.60],[421.5,0.20],[422.5,-0.00],[423.5,-0.20],[424.5,-0.20],[425.5,-0.20],[426.5,-0.00],[427.5,-0.00],[428.5,-0.00],[429.5,-0.00],[430.5,-0.00],[431.5,-0.00],[432.5,-0.00],[433.5,-0.00],[434.5,-0.00],[435.5,-0.00],[436.5,0.20],[437.5,0.20],[438.5,-0.00],[439.5,-0.20],[440.5,-0.20],[441.5,-0.40],[442.5,-0.40],[443.5,-0.20],[444.5,-0.00],[445.5,-0.00],[446.5,-0.00],[447.5,-0.00],[448.5,-0.00],[449.5,-0.00],[450.5,-0.20],[451.5,-0.20],[452.5,-0.40],[453.5,-0.40],[454.5,-0.60],[455.5,-0.40],[456.5,-0.40],[457.5,-0.20],[458.5,-0.10],[459.5,0.30],[460.5,0.30],[461.5,0.30],[462.5,0.30],[463.5,0.20],[464.5,-0.00],[465.5,-0.20],[466.5,-0.20],[467.5,-0.40],[468.5,-0.40],[469.5,-0.60],[470.5,-0.40],[471.5,-0.40],[472.5,-0.20],[473.5,-0.20],[474.5,0.20],[475.5,0.20],[476.5,0.20],[477.5,0.20],[478.5,0.40],[479.5,0.20],[480.5,0.20],[481.5,0.20],[482.5,0.20],[483.5,-0.00],[484.5,-0.00],[485.5,0.20],[486.5,0.20],[487.5,0.20],[488.5,0.20],[489.5,0.20],[490.5,-0.00],[491.5,-0.00],[492.5,-0.00],[493.5,-0.00],[494.5,-0.00],[495.5,-0.00],[496.5,-0.00],[497.5,-0.00],[498.5,-0.00],[499.5,-0.00],[500.5,-0.00],[501.5,-0.00],[502.5,-0.00],[503.5,-0.00],[504.5,-0.00],[505.5,-0.00],[506.5,0.20],[507.5,0.20],[508.5,0.20],[509.5,-0.00],[510.5,0.10],[511.5,-0.10],[512.5,0.10],[513.5,0.10],[514.5,0.40],[515.5,0.10],[516.5,0.10],[517.5,-0.10],[518.5,0.10],[519.5,-0.00],[520.5,0.20],[521.5,0.20],[522.5,0.20],[523.5,-0.20],[524.5,-0.20],[525.5,-0.20],[526.5,-0.20],[527.5,-0.20],[528.5,-0.20],[529.5,-0.00],[530.5,-0.00],[531.5,-0.00],[532.5,-0.00],[533.5,0.20],[534.5,0.20],[535.5,-0.00],[536.5,-0.20],[537.5,-0.20],[538.5,-0.20],[539.5,-0.40],[540.5,-0.00],[541.5,0.20],[542.5,0.40],[543.5,0.40],[544.5,0.20],[545.5,-0.00],[546.5,0.20],[547.5,-0.00],[548.5,-0.20],[549.5,-0.00],[550.5,-0.20],[551.5,-0.40],[552.5,-0.40],[553.5,-0.20],[554.5,-0.20],[555.5,0.20],[556.5,0.20],[557.5,-0.00],[558.5,-0.00],[559.5,0.20],[560.5,-0.00],[561.5,-0.00],[562.5,-0.00],[563.5,-0.00],[564.5,-0.00],[565.5,-0.20],[566.5,-0.40],[567.5,-0.20],[568.5,-0.20],[569.5,-0.40],[570.5,-0.20],[571.5,-0.00],[572.5,0.10],[573.5,0.30],[574.5,0.30],[575.5,0.10],[576.5,0.10],[577.5,-0.00],[578.5,-0.20],[579.5,-0.20],[580.5,-0.00],[581.5,0.10],[582.5,0.10],[583.5,0.10],[584.5,0.10],[585.5,0.10],[586.5,-0.00],[587.5,-0.00],[588.5,-0.20],[589.5,-0.00],[590.5,-0.00],[591.5,0.20],[592.5,0.20],[593.5,0.40],[594.5,0.20],[595.5,0.40],[596.5,0.20],[597.5,0.20],[598.5,0.20],[599.5,0.40],[600.5,0.20],[601.5,0.40],[602.5,0.50],[603.5,0.50],[604.5,0.30],[605.5,0.10],[606.5,-0.30],[607.5,-0.40],[608.5,-0.40],[609.5,-0.40],[610.5,-0.20],[611.5,-0.00],[612.5,-0.00],[613.5,-0.20],[614.5,-0.10],[615.5,-0.10],[616.5,-0.10],[617.5,-0.10],[618.5,0.10],[619.5,-0.00],[620.5,-0.00],[621.5,-0.00],[622.5,-0.00],[623.5,-0.20],[624.5,-0.20],[625.5,-0.20],[626.5,-0.20],[627.5,-0.20],[628.5,-0.00],[629.5,-0.00],[630.5,-0.20],[631.5,-0.20],[632.5,-0.10],[633.5,-0.10],[634.5,-0.10],[635.5,0.10],[636.5,0.10],[637.5,-0.00],[638.5,-0.00],[639.5,-0.00],[640.5,-0.00],[641.5,-0.00],[642.5,0.20],[643.5,-0.00],[644.5,-0.00],[645.5,-0.00],[646.5,-0.20],[647.5,-0.20],[648.5,0.20],[649.5,-0.00],[650.5,0.10],[651.5,0.30],[652.5,0.10],[653.5,-0.10],[654.5,0.10],[655.5,-0.20],[656.5,-0.20],[657.5,-0.20],[658.5,-0.20],[659.5,-0.20],[660.5,-0.00],[661.5,-0.00],[662.5,-0.00],[663.5,-0.00],[664.5,0.10],[665.5,0.10],[666.5,0.10],[667.5,-0.10],[668.5,-0.30],[669.5,-0.40],[670.5,-0.40],[671.5,-0.20],[672.5,-0.00],[673.5,0.20]];dis_datapoints = [];trans_datapoints = [[398,400,'inwards'],[401,415,'inwards']];sec_helix_datapoints = [[51,62],[108,120],[194,200],[201,204],[255,267],[333,335],[390,398],[416,419],[441,445],[493,501]];sec_strand_datapoints = [[11,15],[37,41],[43,49],[65,68],[74,79],[86,91],[99,105],[124,126],[137,143],[149,154],[181,184],[186,191],[208,214],[220,226],[232,238],[245,252],[272,274],[285,290],[296,301],[379,382],[403,408],[449,458],[462,468],[477,484],[530,537],[541,550],[554,562],[585,589],[597,600],[601,607],[612,619],[636,644],[654,662]];acc_exposed_datapoints = [[1,4],[28,28],[52,53],[68,68],[70,72],[82,82],[84,85],[94,96],[108,109],[116,116],[120,120],[124,124],[127,127],[129,129],[131,132],[135,135],[146,146],[157,157],[160,161],[165,166],[170,170],[175,175],[177,177],[195,195],[199,199],[216,218],[230,230],[240,242],[256,257],[260,260],[263,263],[266,267],[269,269],[271,271],[304,304],[309,309],[311,311],[314,314],[318,318],[329,329],[333,333],[337,337],[340,340],[342,342],[346,346],[351,352],[354,355],[365,365],[368,368],[372,374],[386,386],[430,433],[436,436],[439,439],[442,442],[445,446],[449,449],[470,470],[472,472],[475,475],[477,477],[486,486],[509,509],[512,512],[515,515],[526,526],[551,551],[553,553],[565,565],[568,569],[576,576],[578,578],[580,580],[594,594],[600,600],[608,609],[611,611],[626,627],[630,630],[633,633],[645,652],[666,666],[668,668],[670,671],[674,676]];acc_buried_datapoints = [[10,23],[36,36],[38,48],[51,51],[54,55],[57,59],[61,65],[67,67],[75,77],[79,80],[86,90],[97,98],[100,103],[105,105],[110,111],[114,115],[118,118],[122,123],[125,125],[139,140],[142,142],[144,144],[148,148],[150,150],[152,154],[179,182],[184,191],[194,194],[197,198],[200,200],[201,202],[204,210],[213,213],[221,227],[231,237],[245,252],[255,255],[258,259],[261,262],[265,265],[272,272],[274,292],[294,301],[321,321],[323,325],[331,331],[335,336],[369,369],[378,383],[391,397],[399,400],[401,410],[413,420],[422,422],[426,426],[438,438],[440,440],[443,444],[447,448],[450,450],[452,452],[454,454],[456,461],[463,463],[465,465],[467,467],[469,469],[471,471],[473,473],[476,476],[478,478],[480,480],[482,482],[484,484],[489,504],[506,508],[510,511],[514,514],[519,520],[523,524],[527,534],[536,536],[538,544],[546,546],[548,548],[550,550],[554,555],[557,557],[559,559],[561,561],[563,564],[566,566],[570,570],[572,573],[577,577],[582,582],[584,584],[586,588],[590,590],[595,595],[597,597],[599,599],[601,601],[603,605],[607,607],[610,610],[612,615],[617,617],[629,629],[631,632],[634,639],[641,641],[643,643],[654,654],[656,656],[658,660],[662,662],[664,664],[672,672]];flot_plot_options = []; flot_plot_options[0] = {grid: {borderWidth: {top: 0,right: 0,bottom: 0,left: 0}},legend: {show: false},xaxes: [{show: true,min: 0,max: 200,ticks: [[0.5, '1'], [24.5, '25'], [49.5, '50'], [74.5, '75'], [99.5, '100'], [124.5, '125'], [149.5, '150'], [174.5, '175'], [199.5, '200']],tickLength: -5}],yaxes: [{show: true,ticks: [[0, '0'], [4.5,'hydro-<br>phobic&nbsp;&nbsp;'], [-4.5,'hydro-<br>philic&nbsp;&nbsp;']],min: -4.5,max: +4.5,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(100,149,237,1)'}},{show: true,ticks: [[0, ''], [1,'positive<br>&nbsp;charge'], [-1,'negative<br>&nbsp;charge']],position: 'right',min: -1,max: 1,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(255,99,71,1)'}}]};number_of_plots = 4;for ( plot_num = 1 ; plot_num < number_of_plots ; plot_num ++){flot_plot_options[plot_num] = $.extend(true, {} ,flot_plot_options[0]);flot_plot_options[plot_num].xaxes = [{min: plot_num*200,max: (plot_num + 1)*200,ticks: [ [plot_num*200 +  0.5, (plot_num*200 +  1).toString()], [plot_num*200 +  24.5, (plot_num*200 +  25).toString()], [plot_num*200 +  49.5, (plot_num*200 +  50).toString()], [plot_num*200 +  74.5, (plot_num*200 +  75).toString()], [plot_num*200 +  99.5, (plot_num*200 + 100).toString()], [plot_num*200 + 124.5, (plot_num*200 + 125).toString()], [plot_num*200 + 149.5, (plot_num*200 + 150).toString()], [plot_num*200 + 174.5, (plot_num*200 + 175).toString()], [plot_num*200 + 199.5, (plot_num*200 + 200).toString()] ],tickLength: -5}];};try {if( $('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_button').val() =='Show' ){$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_container').css('display','block');$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_button').val('Hide');var description_html = '<div id=\'AutoAnnotator_plot_selectors\'>';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'hydrophobicity_checkbox\' checked=\'checked\'>&nbsp;Moving average over 5 amino acids for hydrophobicity (<img src=\'https://static.igem.org/mediawiki/2013/e/e9/TUM13_hydrophobicity_icon.png\' alt=\'blue graph\' height=\'10\'></img>)';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'charge_checkbox\' checked=\'checked\'>&nbsp;Moving average over 5 amino acids for charge (<img src=\'https://static.igem.org/mediawiki/2013/3/3e/TUM13_charge_icon.png\' alt=\'red graph\' height=\'10\'></img>)';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'dis_checkbox\' checked=\'checked\'>&nbsp;Predicted disulfid bridges (<img src=\'https://static.igem.org/mediawiki/2013/2/28/TUM13_dis_icon.png\' alt=\'yellow circle\' height=\'10\'></img>) with the number of the bridge in the center';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'trans_checkbox\' checked=\'checked\'>&nbsp;Predicted transmembrane helices (<img src=\'https://static.igem.org/mediawiki/2013/7/78/TUM13_trans_icon.png\' alt=\'turquois bars\' height=\'10\'></img>)';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'sec_checkbox\' checked=\'checked\'>&nbsp;Predicted secondary structure: Helices (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_helix_icon.png\' alt=\'violet bars\' height=\'10\'></img>) and beta-strands (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_strand_icon.png\' alt=\'yellow bars\' height=\'10\'></img>)';description_html = description_html + '<br>&nbsp;<input type=\'checkbox\' id=\'acc_checkbox\' checked=\'checked\'>&nbsp;Predicted solvent accessability: Exposed (<img src=\'https://static.igem.org/mediawiki/2013/1/16/TUM13_exposed_icon.png\' alt=\'blue bars\' height=\'10\'></img>) and buried (<img src=\'https://static.igem.org/mediawiki/2013/0/0b/TUM13_buried_icon.png\' alt=\'green bars\' height=\'10\'></img>) residues';description_html = description_html + '<br></div>';$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_explanation').html(description_html);plot_according_to_selectors_1381244514885();$('#AutoAnnotator_container_1381244514885 #AutoAnnotator_plot_selectors').find('input').click(plot_according_to_selectors_1381244514885);}else{$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_container').css('display','none');$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_button').val('Show');$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_explanation').html('');}}catch(err){txt='There was an error with the button controlling the visibility of the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';alert(txt);}};function plot_according_to_selectors_1381244514885(){try{var plot_datasets = [[],[]];if($('#AutoAnnotator_container_1381244514885 #hydrophobicity_checkbox').prop('checked') == true){plot_datasets[0] = { color: 'rgba(100,149,237,1)',data: hydrophobicity_datapoints,label: 'Hydrophobicity',lines: { show: true, fill: true, fillColor: 'rgba(100,149,237,0.1)' },yaxis: 1};}if($('#AutoAnnotator_container_1381244514885 #charge_checkbox').prop('checked') == true){plot_datasets[1] = {color: 'rgba(255,99,71,1)',data: charge_datapoints,label: 'Charge',lines: { show: true, fill: true, fillColor: 'rgba(255,99,71,0.1)' },yaxis: 2};}for (plot_num = 0 ; plot_num < number_of_plots ; plot_num ++){$.plot('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_placeholder'+ plot_num.toString(), plot_datasets, flot_plot_options[plot_num] );}var screen_width = $('canvas.flot-base').width(); var pos_of_first_tick = 46;var pos_of_last_tick = screen_width - 51;var tick_diff = (screen_width - 97)/199;if($('#AutoAnnotator_container_1381244514885 #dis_checkbox').prop('checked') == true){for ( j = 0 ; j < dis_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][0] - 1)*tick_diff - Math.floor((dis_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][1] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][1] - 1)*tick_diff - Math.floor((dis_datapoints[j][1] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');}}if($('#AutoAnnotator_container_1381244514885 #trans_checkbox').prop('checked') == true){for ( j = 0 ; j < trans_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_placeholder' + Math.floor((trans_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_trans\' style=\'width:' + (((trans_datapoints[j][1] - trans_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px ;left:' + ((pos_of_first_tick + (trans_datapoints[j][0] - 1.5)*tick_diff - Math.floor((trans_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if($('#AutoAnnotator_container_1381244514885 #sec_checkbox').prop('checked') == true){for ( j = 0 ; j < sec_helix_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_placeholder' + Math.floor((sec_helix_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_helix\' style=\'width:' + (((sec_helix_datapoints[j][1] - sec_helix_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_helix_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_helix_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < sec_strand_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_placeholder' + Math.floor((sec_strand_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_strand\' style=\'width:' + (((sec_strand_datapoints[j][1] - sec_strand_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_strand_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_strand_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if($('#AutoAnnotator_container_1381244514885 #acc_checkbox').prop('checked') == true){for ( j = 0 ; j < acc_buried_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_placeholder' + Math.floor((acc_buried_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_buried\' style=\'width:' + (((acc_buried_datapoints[j][1] - acc_buried_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_buried_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_buried_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < acc_exposed_datapoints.length ; j++ ){$('#AutoAnnotator_container_1381244514885 #hydrophobicity_charge_placeholder' + Math.floor((acc_exposed_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_exposed\' style=\'width:' + (((acc_exposed_datapoints[j][1] - acc_exposed_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_exposed_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_exposed_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}}catch(err){txt='There was an error while drawing the selected elements for the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';throw(txt);}}</script></html>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 15:02, 8 October 2013

Membrane-anchored Catechol-2,3-dioxygenase (XylE) in RFC[25] N-Part

This part codes for a membrane-anchored version of Catechol-2,3-dioxygenase (XylE) for the chassis Physcomitrella patens. Please see subparts for for further description. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1038
    Illegal BsaI.rc site found at 1956

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)
 AACAATGCCTGGT ... CTCTACAAAACCGGT
 ORF from nucleotide position 5 to 2032 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

101 
201 
301 
401 
501 
601 
MPGEVAWWRPLFLIALMPIGVLSNAEGDALNTTGNKGVMRPGHVQLRVLDMSKALEHYVELLGLIEMDRDDQGRVYLKAWTEVDKFSLVLREADEPGMDF
MGFKVVDEDALRQLERDLMAYGCAVEQLPAGELNSCGRRVRFQAPSGHHFELYADKEYTGKWGLNDVNPEAWPRDLKGMAAVRFDHALMYGDELPATYDL
FTKVLGFYLAEQVLDENGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETWEDLLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVF
CGGDYNYPDHKPVTWTTDQLGKAIFYHDRILNERFMTVLTTGSAWSHPQFEKGENLYFQSGTGPGQPPFPPPPPFTPPPPQTPNGASGENSTGAIAGGVA
AGAALLFAAPAIGFAWWRRRRPIEATGGGSGGGSGTGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTL
TYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
FKIRHNIEDGSVQLADHYQQNTPIGDGPVLSPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKTG*
Sequence features: (with their position in the amino acid sequence, see the list of supported features)
RFC25 scar (shown in bold): 33 to 34, 341 to 342, 362 to 363, 426 to 427, 436 to 437
Strep-tag II: 345 to 352
TEV cleavage site: 354 to 360
Amino acid composition:
Ala (A)46 (6.8%)
Arg (R)30 (4.4%)
Asn (N)27 (4.0%)
Asp (D)46 (6.8%)
Cys (C)5 (0.7%)
Gln (Q)20 (3.0%)
Glu (E)43 (6.4%)
Gly (G)71 (10.5%)
His (H)26 (3.8%)
Ile (I)25 (3.7%)
Leu (L)61 (9.0%)
Lys (K)35 (5.2%)
Met (M)17 (2.5%)
Phe (F)35 (5.2%)
Pro (P)41 (6.1%)
Ser (S)29 (4.3%)
Thr (T)43 (6.4%)
Trp (W)11 (1.6%)
Tyr (Y)24 (3.6%)
Val (V)41 (6.1%)
Amino acid counting
Total number:676
Positively charged (Arg+Lys):65 (9.6%)
Negatively charged (Asp+Glu):89 (13.2%)
Aromatic (Phe+His+Try+Tyr):96 (14.2%)
Biochemical parameters
Atomic composition:C3370H5127N911O998S22
Molecular mass [Da]:75077.6
Theoretical pI:5.53
Extinction coefficient at 280 nm [M-1 cm-1]:96260 / 96573 (all Cys red/ox)
Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges 
Codon usage
Organism:E. coliB. subtilisS. cerevisiaeA. thalianaP. patensMammals
Codon quality (CAI):good (0.73)good (0.73)good (0.65)good (0.74)excellent (0.82)good (0.74)
Alignments (obtained from PredictProtein.org)
SwissProt:P06622 (100% identity on 306 AAs), P42212 (98% identity on 237 AAs)
TrEML:D2XKL2 (100% identity on 306 AAs), Q38Q96 (100% identity on 293 AAs), Q7AXV1 (100% identity on 306 AAs), Q7BH44 (100% identity on 306 AAs), Q9L780 (99% identity on 306 AAs), B6UPG7 (98% identity on 237 AAs), Q71RY9 (98% identity on 237 AAs), Q8GHE2 (98% identity on 237 AAs)
PDB:1mpy (100% identity on 306 AAs), 2y0g (98% identity on 226 AAs)
Predictions (obtained from PredictProtein.org)
Subcellular Localization (reliability in brackets)
Archaea:cytosol (100%)
Bacteria:cytosol (51%)
Eukarya:cytosol (40%)
Gene Ontology (reliability in brackets)
Molecular Function Ontology: -
Biological Process Ontology:GO:0019439 (42%), GO:0055114 (29%)
 
Predicted features:
Disulfid bridges: -
Transmembrane helices:398 to 415 going inwards
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation.
If you have any questions, comments or suggestions, please leave us a comment.