Difference between revisions of "Part:BBa K1202116:Experience"

 
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To observe inducible promoters response, we co incubated two different bacteria. The first bacteria culture is expressing enzyme system and the second culture is including inducible promoter. At the experiment, we incubated them seperately for 16 hours and mixed them into one flask and 4 hour later, we add 0,1 mM SAH (with 10x PBS containing %1 BSA). Incubate with SAH for one day and, took 2 ml of liquid culture to santrifuge tube.Centrifuged it. We saw the red color at the pellet as you see below.
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[[File:Tvymn.jpg]]
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So, it means the enzyme system is working and inducible promoter is succesfully induced from AI-2.
  
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Discussion
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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We were searching for inducible system that, getting activated with cancer cell antigen precense. As an activating molecule, autoinducer2 activates LsrR-LsrK promoter system for express the killer protein by bacteria.
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how you used this part and how it worked out.
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Our co-incubation liquid cultures’ santrifuged tubes are seemin red colored.We added rfp gene to continue of inducible promotor gene and rfp is marker of activating. So this co incubation result significantly shows us promoter system is activating in the precense of autoindcer-2.
  
 
===Applications of BBa_K1202116===
 
===Applications of BBa_K1202116===

Revision as of 08:58, 8 October 2013

To observe inducible promoters response, we co incubated two different bacteria. The first bacteria culture is expressing enzyme system and the second culture is including inducible promoter. At the experiment, we incubated them seperately for 16 hours and mixed them into one flask and 4 hour later, we add 0,1 mM SAH (with 10x PBS containing %1 BSA). Incubate with SAH for one day and, took 2 ml of liquid culture to santrifuge tube.Centrifuged it. We saw the red color at the pellet as you see below. Tvymn.jpg

So, it means the enzyme system is working and inducible promoter is succesfully induced from AI-2.

Discussion We were searching for inducible system that, getting activated with cancer cell antigen precense. As an activating molecule, autoinducer2 activates LsrR-LsrK promoter system for express the killer protein by bacteria. Our co-incubation liquid cultures’ santrifuged tubes are seemin red colored.We added rfp gene to continue of inducible promotor gene and rfp is marker of activating. So this co incubation result significantly shows us promoter system is activating in the precense of autoindcer-2.

Applications of BBa_K1202116

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