Difference between revisions of "Part:BBa K1152016"
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In the multiple sequence alignment showed below, this variant of indC is named synT1. | In the multiple sequence alignment showed below, this variant of indC is named synT1. | ||
| − | [[image:IndPD_Fig4.png|800px|Multiple Sequence Alignment of the synthetic T-domains we introduced | + | [[image:IndPD_Fig4.png|800px|thumb|center|'''Figure 1: Multiple Sequence Alignment of the synthetic T-domains we introduced into indC''' The label ''indC'' reflects the T-domain of indC as predicted by the Pfam domain prediction (pfam.sanger.ac.uk). For a detailled description of the method we used to create the synthetic T-domains, please see [http://2013.igem.org/Team:Heidelberg/Project/Indigoidine our wiki]]] |
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Revision as of 21:51, 6 October 2013
IndC Indigoidine Synthetase device synT1 (engineered)
This engineered version of indC BBa_K11520013 contains a synthetic T-domain instead of its native T-domain. It still is capable to produce the blue pigment indigoidine as OD-measurements showed.
In the multiple sequence alignment showed below, this variant of indC is named synT1.
Figure 1: Multiple Sequence Alignment of the synthetic T-domains we introduced into indC The label indC reflects the T-domain of indC as predicted by the Pfam domain prediction (pfam.sanger.ac.uk). For a detailled description of the method we used to create the synthetic T-domains, please see [http://2013.igem.org/Team:Heidelberg/Project/Indigoidine our wiki]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4087
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1467
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2730
Figure 2 shows the analytical restiction digest we performed for part validation:
