Difference between revisions of "Part:BBa K1152010"

 
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<partinfo>BBa_K1152010 short</partinfo>
 
<partinfo>BBa_K1152010 short</partinfo>
  
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svp encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes.
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[[File:Heidelberg2013_PPtase_svp.png|220px|thumb|left|'''Figure 1:''' Phenotypes of TOP10 producig indigoidine. As negative control untransformed TOP10 were used.]]
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Svp (Figure 1) encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (see Figure 2).
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[[File:Heidelberg2013_PPtase_mechanism.png|280px|thumb|'''Figure 2:''' General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.]]
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Figure 3 shows the analytical restiction digest we performed for part validation:
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[[File:Heidelberg2013_BBa_testdigest_CDS.png|280px|thumb|center|'''Figure 2:''' Restriction digest with EcoRI, XbaI, SpeI, PstI.]]
  
 
<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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Svp is a PPTase often associated with activating the indigoidine synthetase bpsA [Takahashi, 2007].
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1152010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1152010 SequenceAndFeatures</partinfo>
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===References===
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* Takahashi, H., Kumagai, T., Kitani, K., Mori, M., Matoba, Y., & Sugiyama, M. (2007). Cloning and Characterization of a Streptomyces Single Module Type Non-ribosomal Peptide Synthetase Catalyzing a Blue Pigment Synthesis *, 282(12), 9073–9081. doi:10.1074/jbc.M611319200
  
  

Latest revision as of 18:10, 6 October 2013

Svp 4'-Phosphopanthetheinyl-transferase


Figure 1: Phenotypes of TOP10 producig indigoidine. As negative control untransformed TOP10 were used.

Svp (Figure 1) encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes (see Figure 2).

Figure 2: General mechanism of activation the activation of NRP Synthetases from apo- to holo-form.

Figure 3 shows the analytical restiction digest we performed for part validation:

Figure 2: Restriction digest with EcoRI, XbaI, SpeI, PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 173
    Illegal NgoMIV site found at 413
    Illegal NgoMIV site found at 663
    Illegal AgeI site found at 573
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 366

References

  • Takahashi, H., Kumagai, T., Kitani, K., Mori, M., Matoba, Y., & Sugiyama, M. (2007). Cloning and Characterization of a Streptomyces Single Module Type Non-ribosomal Peptide Synthetase Catalyzing a Blue Pigment Synthesis *, 282(12), 9073–9081. doi:10.1074/jbc.M611319200