Difference between revisions of "Part:BBa K823039:Design"
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===Source=== | ===Source=== | ||
− | Plasmid [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=306491148 pGFPamy] | + | Plasmid [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=306491148 pGFPamy] or maybe not... sorry this is going to be re-checked. |
===References=== | ===References=== | ||
[http://www.ncbi.nlm.nih.gov/pubmed/20600285 Bisicchia(2010)] | [http://www.ncbi.nlm.nih.gov/pubmed/20600285 Bisicchia(2010)] |
Revision as of 10:10, 6 October 2013
gfpmut1 (B. subtilis optimized + Freiburg standard)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 197
Design Notes
We designed this part in the Freiburg standard for N- and C-terminal fusions.
Source
Plasmid [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=306491148 pGFPamy] or maybe not... sorry this is going to be re-checked.
References
[http://www.ncbi.nlm.nih.gov/pubmed/20600285 Bisicchia(2010)]