Difference between revisions of "Part:BBa K1113701:Experience"
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We test the targeting sequence of the RuBisCO-GFP (the large and short subunit) in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 ''E. coli'' strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes). | We test the targeting sequence of the RuBisCO-GFP (the large and short subunit) in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 ''E. coli'' strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes). | ||
[[File:Team_UC_Microscopioconfocal.jpg]]<br> | [[File:Team_UC_Microscopioconfocal.jpg]]<br> | ||
| − | Figure 1. Confocal Microscopy. Brightfield, GFP filter, RFP filter and merge are shown | + | Figure 1. Confocal Microscopy. Brightfield, GFP filter, RFP filter and merge are shown. |
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| + | As observed in Figure 1, two of our constructs display co-localization of green and red fluorescence. Finally we quantify the amount of colocalization of our targeting sequence and obtained (Table 1) that the construct that had the grearest co-localization was C.LG, which is the large subunit of RuBisCO with GFP attached to the carboxil terminal end of the enzime. <br> | ||
| + | [[File:Nuevaimagen.jpg]]<br> | ||
| + | Table 1. Quantify of the colocalization. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 22:11, 5 October 2013
We test the targeting sequence of the RuBisCO-GFP (the large and short subunit) in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 E. coli strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes).
Figure 1. Confocal Microscopy. Brightfield, GFP filter, RFP filter and merge are shown.
As observed in Figure 1, two of our constructs display co-localization of green and red fluorescence. Finally we quantify the amount of colocalization of our targeting sequence and obtained (Table 1) that the construct that had the grearest co-localization was C.LG, which is the large subunit of RuBisCO with GFP attached to the carboxil terminal end of the enzime.
Table 1. Quantify of the colocalization.
User Reviews
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