Difference between revisions of "Part:BBa K1230000:Experience"
(→User Reviews) |
|||
| Line 15: | Line 15: | ||
<I>Username</I> | <I>Username</I> | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
| − | + | ||
| + | We were able to characterise this part, since we found a measurable phenotype (kanamycin resistance). | ||
| + | |||
| + | |||
| + | We also characterised it using Kanamycin �Minimum Inhibitory Concentration� (MIC) finding a MIC with MarA overexpression: 5 µg/ml and a wild-type MIC: 1 µg/ml | ||
| + | |||
| + | |||
| + | |||
| + | https://static.igem.org/mediawiki/igem.org/8/8b/U102_Fig2.png | ||
| + | |||
| + | A test was performed with three different antibiotics and different IPTG concentrations. | ||
| + | The part seems to make the host sensible to Tetracyclin (maybe because it's on Cm plasmid, and both antibiotics act on similar ways), it seems not to have an effect on Ampicilin and it shows a relevant effect with Kanamycin. Which is further explored in the next figure. | ||
| + | |||
| + | https://static.igem.org/mediawiki/igem.org/2/21/U101_Fig3.png | ||
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K1230000 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1230000 EndReviews</partinfo> | ||
Revision as of 22:08, 5 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1230000
User Reviews
UNIQ751866b2cbaafff7-partinfo-00000000-QINU UNIQ751866b2cbaafff7-partinfo-00000001-QINU