Difference between revisions of "Part:BBa K1130000:Design"

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Synthetic Promoter derived from rRNA promoters extracted from the L. plantarum WCFS1 genome.
 
Synthetic Promoter derived from rRNA promoters extracted from the L. plantarum WCFS1 genome.
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Cloned and expression in L.plantarum and E.coli, measured activity with GFP ligation.
+
Cloned and expression in L.plantarum and E.coli, measured activity with GFP ligation
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[[File:P1 expression.jpg]]
  
 
===References===
 
===References===
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Rud I, Jensen PR, Naterstad K, Axelsson L. (2006) A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum.

Latest revision as of 22:07, 5 October 2013

Constitutive promoter 1 L.plantarum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Biobricks prefix and subffix was add to the sequence. Synthetic Promoter derived from rRNA promoters extracted from the L. plantarum WCFS1 genome.

Source

Synthetic Promoter derived from rRNA promoters extracted from the L. plantarum WCFS1 genome. Cloned and expression in L.plantarum and E.coli, measured activity with GFP ligation P1 expression.jpg

References

Rud I, Jensen PR, Naterstad K, Axelsson L. (2006) A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum.