Difference between revisions of "Part:BBa K1113701:Experience"
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We test the targeting sequence of the RuBisCO-GFP (the large and short subunit) in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 ''E. coli'' strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes). | We test the targeting sequence of the RuBisCO-GFP (the large and short subunit) in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 ''E. coli'' strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes). | ||
| − | [[File:Team_UC_Microscopioconfocal.jpg]] | + | [[File:Team_UC_Microscopioconfocal.jpg]]<br> |
Figure 1. Confocal Microscopy. Brightfield, GFP filter, RFP filter and merge are shown | Figure 1. Confocal Microscopy. Brightfield, GFP filter, RFP filter and merge are shown | ||
Revision as of 22:01, 5 October 2013
Applications of BBa_K1113701
We test the targeting sequence of the RuBisCO-GFP (the large and short subunit) in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 E. coli strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes).
Figure 1. Confocal Microscopy. Brightfield, GFP filter, RFP filter and merge are shown
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