Difference between revisions of "Part:BBa K1113701:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
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===Applications of BBa_K1113701===
 
===Applications of BBa_K1113701===
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The final goal of our constructs it is the delivery of the GFP into the Carboxysome, to observe this we co-transformed TOP10 with both the Carboxysome BioBrick (Bba_K1113100) + RFP (Bba_E1010) and our constructs to observe if they colocalized.
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We test the targeting sequence of the RuBisCO-GFP in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 ''E. coli'' strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes).
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[[File:Team_UC_Microscopioconfocal.jpg]]
 
[[File:Team_UC_Microscopioconfocal.jpg]]
  

Revision as of 21:54, 5 October 2013

Applications of BBa_K1113701

We test the targeting sequence of the RuBisCO-GFP in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 E. coli strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes).

Team UC Microscopioconfocal.jpg

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