Difference between revisions of "Part:BBa K1232004"
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HO1 and PcyA genes are required for chromophore synthesis. PLPCB protein groups transforms heme compounds into phycocyanobilin (PCB).
 
HO1 and PcyA genes are required for chromophore synthesis. PLPCB protein groups transforms heme compounds into phycocyanobilin (PCB).
  
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===Usage and Biology===
 
===Usage and Biology===
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For the biology of this part refer to the [http://2013.igem.org/Team:Washington/LightSensing#Systems Washington 2013 wiki page.]
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[[Image:UW_Uwvtabor.png|410px|left|thumb|Native versus Biobrick systems over time.]]
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The first pair of lines in green represent the fluorescence time course of a dual transformation of the native plasmids supplied by the Tabor Lab at Rice University in accordance to the light testing protocol described by the University of Washington iGEM 2013 team on their wiki. There is a measurable difference in GFP expression between the green light and dark conditions.
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The second pair of lines in red represent the same experiment but using this [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1232000 BBa_K1232000]  to express the light sensing proteins and the native plasmid for expression of chromophore expression. There remains a measurable difference in GFP expression between green light and dark conditions.
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The third pair of lines in blue represent, again, the same experimental setup but using this biobrick (BBa_K1232004)to express the chromophore producing proteins and the native plasmid for expressing light sensing proteins. Again, there is a measurable difference in GFP expression between green light and dark conditions.
  
 
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Revision as of 20:38, 5 October 2013

Cyanobacterial chromophore = Para/lac-RBS-Ho1-RBS-PcyA

HO1 and PcyA genes are required for chromophore synthesis. PLPCB protein groups transforms heme compounds into phycocyanobilin (PCB).


Usage and Biology

For the biology of this part refer to the [http://2013.igem.org/Team:Washington/LightSensing#Systems Washington 2013 wiki page.]

Native versus Biobrick systems over time.


The first pair of lines in green represent the fluorescence time course of a dual transformation of the native plasmids supplied by the Tabor Lab at Rice University in accordance to the light testing protocol described by the University of Washington iGEM 2013 team on their wiki. There is a measurable difference in GFP expression between the green light and dark conditions.


The second pair of lines in red represent the same experiment but using this BBa_K1232000 to express the light sensing proteins and the native plasmid for expression of chromophore expression. There remains a measurable difference in GFP expression between green light and dark conditions.


The third pair of lines in blue represent, again, the same experimental setup but using this biobrick (BBa_K1232004)to express the chromophore producing proteins and the native plasmid for expressing light sensing proteins. Again, there is a measurable difference in GFP expression between green light and dark conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 458
    Illegal BamHI site found at 1222
    Illegal XhoI site found at 351
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 458
    Illegal NgoMIV site found at 1549
  • 1000
    COMPATIBLE WITH RFC[1000]